Staphylococcus Aureus Nasal Carriage
Conditions
Brief summary
The aim of this study was to compare the prevalence of nasal carrying of SA ST398 strains in community and hospital settings, identify the factors associated with colonization of SA ST398, determine the distribution of imported and acquired cases among SA ST398 hospital cases and characterize the genetic structure of the ST398 SA population involved in colonization.
Detailed description
Staphylococcus aureus (SA) is a ubiquitous bacterium, part of the human commensal flora. The mucosa of the anterior nasal cavities represents the preferred site of colonization of SA. The SA ST398 clone, belonging to CC 398, was initially described as a zoonotic agent initially identified in France and the Netherlands. In fact, ST398 strains are separated into two distinct populations: a methicillin-resistant SA population (SAMR) with notion of contact with animals and a more virulent methicillin-sensitive SA population (SAMS) than its counterpart, isolated from serious human infections and without notion of exposure to farm animals. Recently, we have shown that, in SA bacteremia, all strains of SA ST398 are sensitive to methicillin and that the prevalence of ST398 among all SAMS responsible for bacteremia at Besançon University Hospital increased between 2009 and 2014, increased from 4 to 15%. However, we were unable to study nasal colonization to support the mode of acquisition of SA ST398 (community vs. healthcare acquisition).
Interventions
PROCEDURENasal swab
In hospitalized patiens, a nasal swab will be performed within the first 48 hours and on the 7th day. In community patient, only one swab will be performed
Sponsors
Centre Hospitalier Universitaire de Besancon
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
For community patients : Inclusion Criteria: * the selection criteria for blood donation * age ≥ 18 years old * No refusal of the person
Exclusion criteria
* None For hospitalized patients : Inclusion Criteria: * age ≥ 18 years old * hospitalization in one of the services participating in the study * no refusal of the person
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of patients nasal carrying of SA ST398 strains in community and hospital settings | One day | Number of patients with SA ST398 positive nasal swab in community will be compared with number of patients with SA ST398 positive nasal swab in hospital |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Number of patietns with specific Clinical and demographic data associated with nasal carrying of SA ST398 | One day | Clinical and demographic (sex, age, gender, comorbidities, tabaco...) data will be compared between patients with SA ST398 positive nasal swab and SA ST398 negative nasal swab |
| Number of patients with SA ST398 positive nasal swab to seven days of hospitalization | 7 days | Number of patients with SA ST398 positive nasal swab to seven days of hospitalization will be compared with the number of patients with SA ST398 positive nasal swab within the first 48 hours of hospitalization |
| Number of virulence gene in SA ST 398 strains | One day | Presence of virulence gene in SA ST 398 strains |
Countries
France
Outcome results
None listed