Arterial Occlusive Diseases, Arteriosclerosis, Atherosclerosis, Cardiovascular Diseases, Glucose Metabolism Disorders, Hyperinsulinism, Insulin Resistance, Metabolic Disease, Metabolic Syndrome X, Vascular Diseases
Conditions
Brief summary
The purpose of this study is to investigate the effect of interrupting prolong sedentary behavior with interval exercise on postprandial metabolism following a high fat glucose tolerance test.
Detailed description
All subjects complete two trials in a randomized crossover design, with each trial occurring over 4 days with a minimum of seven days between trials. The first two days of each trial serve as a control period allowing for familiarization and the control of physical activity and calorie consumption prior to the intervention. Following each control period, subjects then perform one of the interventions on Day 3. The interventions consist of either eight-hours of prolonged sitting, or eight hours of sitting interrupted every hour by five sprints lasting 4 seconds each using the interval exercise. On day 4, all subjects will undergo a high fat glucose tolerance test to determine the body's ability to clear triglycerides from the blood as well as insulin/glucose response and substrate oxidation. Blood samples will be assayed for the previously mentioned substances and postprandial gas collection will be analyzed via indirect calorimetry.
Interventions
BEHAVIORALProlonged sitting without exercise
Subjects will have a 2-day control period during which step count and diet will be controlled for and recreated for the other trial. Following this 2-day period, they will undergo the prolonged sitting trial. Day 4 will consist of the lipid tolerance test to determine the ability of the body to clear triglycerides.
BEHAVIORALProlonged sitting with interval exercise
Subjects will have a 2-day control period during which step count and diet will be controlled for and recreated for the other trial. Following this 2-day period, they will undergo a prolonged sit which with will be broken up every hour on the hour by a small bout of interval exercise. Day 4 will consist of the lipid tolerance test to determine the ability of the body to clear triglycerides.
Sponsors
University of Texas at Austin
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
\-
Exclusion criteria
* cardiovascular problems (e.g. pre-existing heart issues, coronary artery disease, hypertension, etc.) * respiratory problems * musculoskeletal problems that prevent prolonged sitting or exercise * susceptibility to fainting * Pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Plasma Triglycerides | 6-hours | Areas under the curve for triglyceride concentration will be calculated for hourly samples from a 6-hour lipid tolerance test |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Plasma Insulin | 6-hours | Areas under the curve for insulin concentration will be calculated for hourly samples from a 6-hour lipid tolerance test |
| Plasma Glucose | 6-hours | Areas under the curve for glucose concentration will be calculated for hourly samples from a 6-hour lipid tolerance test |
Countries
United States
Outcome results
None listed