Adiposity, Estrogen Deficiency, Follicle-Stimulating Hormone Deficiency, Hormone Deficiency, Menopause Surgical
Conditions
Brief summary
The overarching aims of this study are to: 1. Characterize the rate of in vivo adipogenesis, and changes in adipose tissue gene and protein expression, in the scABD and scFEM depots of women undergoing surgical menopause (↓E2, ↑FSH). 2. Characterize the rate of in vivo adipogenesis, and changes in adipose tissue gene and protein expression, in the scABD and scFEM depots of women undergoing gonadal suppression (↓E2, ↓FSH).
Detailed description
This is a cross-sectional study where two groups of premenopausal women (ages 18-50 y) will be enrolled in a parallel arm study: * Arm 1 (Surgical Menopause): up to 6 women undergoing laparoscopic, elective bilateral oophorectomy \[Site: Pennington Biomedical Research Center\]. * Arm 2 (Pharmacology-Induced Menopause): up to 6 women undergoing gonadal suppression via leuprolide acetate (Lupron \[AbbVie Inc.\]) \[Site: UC-Denver\]. We will compare each arm of women to non-oophorectomized, premenopausal women (controls) with normal menstrual cycles (Apple&Pear study; NCT01748994; PI: Ravussin) selectively matched (1:2) for age and BMI. The Apple&Pear study uses the same in vivo adipogenesis labeling protocol, with similar age and BMI criteria, as the proposed study.
Interventions
DRUG'Drug-Induced Menopause' Group
Women undergoing gonadal suppression via leuprolide acetate (Lupron \[AbbVie Inc.\]) will be enrolled at the University of Colorado-Denver.
PROCEDURE'Surgical Menopause' Group
Women undergoing laparoscopic oophorectomy surgery ('surgical menopause') will be enrolled at Pennington Biomedical.
Sponsors
University of Colorado, Denver
University of Alabama at Birmingham
Pennington Biomedical Research Center
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy female * Ages 18-50 y * Planning to have either a laparoscopic bilateral oophorectomy or a laparoscopic unilateral oophorectomy (which would result in no remaining ovaries) * Are willing to drink heavy water (2H2O) over an 8-week period * Medically cleared for participation in the study by OB/GYN and Medical Investigator * Are willing to have blood and fat tissue stored for future use
Exclusion criteria
* Meet either of the following criteria: * Have all 3 of the major menopause-related symptoms \[hot flashes, mood swings, insomnia (trouble sleeping)\] * Have 2 of the major menopause-related symptom combinations \[hot flashes and mood swings, or hot flashes and insomnia (trouble sleeping)\] * Unstable weight in the last 3 months \[gain or loss \>7 lb (or 3.2 kg)\] * History of clinically diagnosed diabetes or a fasting blood glucose \>126 mg/dL * Chronic use of systemic glucocorticoids, antipsychotic/antidepressant medications, thiazolidinediones and other medications that cause clinically significant weight gain, weight loss or are known to make changes in fat cell number/size \* * Previous bariatric surgery (or other surgeries) for obesity or weight loss (\< 3 years ago) * Use of over the counter or prescription weight loss products * History of metabolic diseases (other than diabetes) * History of neurological disease * History of cardiovascular disease (or other chronic diseases) * Pregnant, planning to become pregnant, or breastfeeding * Use of hormone replacement therapy * Unwilling to discontinue any form or hormonal therapy (e.g., contraceptives including birth control pills, vaginal ring, injections, implant, or skin patch; hormonal supplements, etc.) upon enrollment (after the Screening Visit). * Inconsistent use of medications listed above will be evaluated and left up to the discretion of the Medical Investigator to evaluate safety.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Rate of in vivo adipogenesis (via deuterium-enrichment of adipose tissue DNA) | Change from baseline in enrichment of DNA of adipose cells with deuterium at 8 weeks | Deuterium from the deuterium-labeled water is incorporated into the newly-synthesized DNA of newly-formed fat cell precursor cells through cell replication. The latter carry over the label when they become fat cells through differentiation. Enzymatic digestion of the fat tissue isolates the individual cells constituting the fat tissue. Centrifugation of the cell suspension allows the separation of fat cells into a floating layer and a pellet comprised of stromal-vascular cells including the fat cell precursor cells and small fat cells. As the fat cell precursor cells and small adipocytes have the property to attach quickly to plastic surfaces of culture dishes, a brief culturing of the stromal-vascular cells sorts these cells from the remaining cells. Thus, measuring the deuterium-enrichment of DNA from plastic-adherent stromal-vascular cells indicates the rate of in vivo formation of new mature fat cells and pre-adipocytes, a process collectively termed adipogenesis. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Body composition (by Dual-energy X-ray Absorptiometry (DXA)) | Change from baseline in body composition at 8 weeks post-surgery | Fat mass, fat-free mass, and percent body fat will be assessed using a whole-body scanner GE iDXA. |
| Size of adipocytes | Change from baseline in size of adipocytes at 8 weeks post-surgery | Fat cell size will be determined using osmium fixation of the lipids and measurement of their diameter with Coulter Counter followed by calculation of fat cell volume. The mean lipid content of fat cells will be calculated by multiplying the fat cell volume by the density of triolein (0.915). |
| Number of adipocytes | Change from baseline in number of adipocytes at 8 weeks post-surgery | Fat cell number will be estimated by dividing the volume of adipose tissue depot of interest to the mean fat cell volume or the fat mass of the depot to the mean lipid content in fat cell. |
| Adipose tissue gene and protein expression | Changes from baseline in gene and protein expression at 8 weeks post-surgery | Expression levels of genes and proteins involved in adipocyte expansion and function (ERα, PPARγ2, C/EBPα, aromatase, adiponectin, and LPL), extracellular matrix remodeling and fibrosis (COL6(a1, a2, a3), COL4a1, and TGFβ), and inflammation (IL-6 and TNFα) will be assessed. |
Countries
United States
Outcome results
None listed