Sepsis Syndrome
Conditions
Brief summary
The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.
Detailed description
In this study, the investigators will collect the peripheral blood of 1,3,7,14 day after diagnose of sepsis. Then, the concentration of branched-chain amino acids(BCAAs) in monocytes and plasma, and the expression of BCAAs metabolic enzymes in monocytes will be determined. Additionally, the inflammatory cytokines, e.g., Interleukin-1β (IL-1β), Tumor necrosis factor-α (TNF-α), Interleukin-10 (IL-10）, the chemokines macrophage inflammatory proteins 1α and 1β (MIP-1α and 1β) in the peripheral blood and the phagocytic function of monocytes will also be detected. The current project was designed to examine the metabolic level of branched-chain amino acids in plasm and monocyte/macrophage, and its role in immune dysfunction during sepsis.
Interventions
OTHERMetabolic level of BCAAs in monocytes and macrophages
Metabolic level of BCAAs in monocytes and macrophages
Sponsors
West China Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* patients diagnosed with sepsis, with or without type 2 diabetes * healthy volunteers
Exclusion criteria
* patients with immunodeficient diseases * patients who accept glucocorticoid or immunosuppressant * patients with an age younger than 18 years * patients who cannot provide the informed consent
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Intracellular levels of branched-chain amino acids in monocytes | up to 14 day after diagnose of sepsis | BCAAs Assay Kit will be used to measure the intracellular levels of BCAAs in monocytes. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| The expression of branched-chain amino acid aminotransferase in monocytes | 1,3,5,7,10,14 day after diagnose of sepsis | The expression of branched-chain amino acid aminotransferase in monocytes will be determined by qPCR and western blot. |
| The expression of branched-chain α-ketoacid dehydrogenase in monocytes | 1,3,5,7,10,14 day after diagnose of sepsis | The expression of branched-chain α-ketoacid dehydrogenase in monocytes will be determined by qPCR and western blot. |
| The expression of PP2Cm in monocytes | 1,3,5,7,10,14 day after diagnose of sepsis | The expression of mitochondrial-targeted phosphatase, PP2Cm in monocytes will be determined by qPCR and western blot. |
| The plasma level of IL-1β | 1,3,5,7,10,14 day after diagnose of sepsis. | The plasma level of IL-1β will be determined by ELISA kit. |
| Plasma levels of branched-chain amino acids | 1,3,5,7,10,14 day after diagnose of sepsis | BCAAs Assay Kit will be used to measure the plasma levels of BCAAs. |
| The plasma level of IL-10 | 1,3,5,7,10,14 day after diagnose of sepsis. | The level of IL-10 will be determined by ELISA kit. |
| The plasma level of chemokine MIP-1α. | 1,3,5,7,10,14 day after diagnose of sepsis. | The plasma level of chemokine MIP-1α will be determined by ELISA kit. |
| The plasma level of chemokine MIP-1β. | 1,3,5,7,10,14 day after diagnose of sepsis. | The plasma level of chemokine MIP-1β will be determined by ELISA kit. |
| The phagocytic index of monocytes. | 1,3,5,7,10,14 day after diagnose of sepsis. | The phagocytic index of monocytes will be determined by a E.coli Phagocytosis Assay Kit. |
| The plasma level of TNF-α | 1,3,5,7,10,14 day after diagnose of sepsis. | The plasma level of TNF-α will be determined by ELISA kit. |
Outcome results
None listed