Healthy
Conditions
Keywords
self-reported healthy adults, age 40-70, BMI > 25
Brief summary
The investigators have studied the acute interactions of polyphenols and NDC in a single meal in a human study containing tomatoes, lovage & onions, with and without inulin. The investigators now wish to study the longer-term interactions of these foods over 6 weeks as well as their impact on biomarkers of human health, the gut microbiome, human proteome and markers of risk of chronic disease. This may allow better targeted evaluation of foods which influence health through the gut microbiome.
Detailed description
Polyphenol rich plant foods have been associated with several health benefits but their bioavailability is generally low. The majority of plant polyphenols are poorly absorbed in the small intestine and enter the colon where the colonic microbiota metabolise them to release a range of phenolic acids, which are now thought to be the main bioactive components related to the reduction in disease risk. Very little is known about the impact of other constituents of the diet on the metabolism and bacterial catabolism of these polyphenols. Colonic bacteria are key agents in the release of the bioactive molecules from polyphenols but also ferment non-digestible carbohydrates (NDC) such as inulin to short chain fatty acids. It is likely that there are key interactions in the colonic bacterial metabolism of NDC and phenolics. The investigators hypothesize that combination of polyphenolics (in onions, tomatoes and lovage) with inulin (NDC) will increase the urinary output of bioactive phenolic acids over a 6 week feeding trial and impact on biomarkers of cardiometabolic health.
Interventions
OTHERTomato, onion and lovage soup (TOL)
Soup
OTHERTOL soup with inulin
Soup
Sponsors
Scottish Universities Environmental Research Centre
University of Glasgow
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Masking description
soups in plain tins and labelled A or B. Code held by person not part of study team.
Intervention model description
This intervention study will be carried out using a parallel design, with three arms (foods containing polyphenol+NDC, foods with polyphenol alone, and habitual diet control)
Eligibility
Sex/Gender
ALL
Age
40 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Generally healthy * BMI\>=25 * Not on antibiotics within 3 months of starting of study * No medication likely to influence gut function, colonic bacterial activity.
Exclusion criteria
* Antibiotics * Pregnancy * lactation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change from baseline of total amount of phenolic acids (umoles/24 hours) in urine (3 wk) | 3 weeks | Profile and amounts of phenolic acids in urine derived from the colonic bacterial metabolism of polyphenols |
| Change from baseline of total amount of phenolic acids (umoles/24 hours) in urine (6 wk) | 6 weeks | Profile and amounts of phenolic acids in urine derived from the colonic bacterial metabolism of polyphenols |
Countries
United Kingdom
Outcome results
None listed