Hypospadias
Conditions
Keywords
hypospadias, prepuce, HOXA13, FGFR2, TGF beta
Brief summary
The aim of this study is to compare prepuce tissue samples from healthy male children who applied for circumcision and children with isolated hypospadias. Estrogen receptor 1 (ER1), androgen receptor (AR), FGFR2, transforming growth factor beta (TGF beta) and HOXA13 gene expression in the prepuce is planned to investigate via polymerase chain reaction (PCR) method. Mucosal vascular and nerve densities, and extracellular matrix density of laminin and collagen protein is planned to investigate via immunohistochemical examination.
Interventions
None listed
Sponsors
Trakya University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
MALE
Age
No minimum to 18 Years
Healthy volunteers
Yes
Inclusion criteria
* To apply for circumcision, not to have any genitourinary abnormalities/conditions for control group. * To have isolated hypospadias for study group.
Exclusion criteria
* To have genitourinary surgery before.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Genetic analysis 1 | 1 year | Androgen receptor (AR) gene PCR analysis |
| Genetic analysis 2 | 1 year | Estrogen receptor 1 (ER1) gene PCR analysis |
| Genetic analysis 3 | 1 year | FGFR2 gene PCR analysis |
| Genetic analysis 4 | 1 year | TGF beta gene PCR analysis |
| Genetic analysis 5 | 1 year | HOXA13 gene PCR analysis |
| Immunohistochemical study 1 | 1 year | Mucosal vessel density is planned to determine via CD34 |
| Immunohistochemical study 2 | 1 year | Mucosal nerve density is planned to determine via PGP 9.5 |
| Histological study 1 | 1 year | Laminin is planned to determine in extracellular matrix via light microscope |
| Histological study 2 | 1 year | Collagen is planned to determine in extracellular matrix via light microscope |
Countries
Turkey (Türkiye)
Outcome results
None listed