Diminished Ovarian Reserve, Invitro Fertilization, Oocyte
Conditions
Keywords
follicular flushing, double lumen needle
Brief summary
Retrieval of an oocyte from a follicle relies on the release of the cumulus-oocyte-complex (COC) from the follicular wall into the lumen, thereby allowing aspiration by a needle. Flushing the follicle with multiple aspirations from the same follicle is used as a means to increase the ratio of COC per aspirated follicle. Data showed that follicular flushing is not superior to direct aspiration either in normally responding patients or in poor responders undergoing in vitro fertilization (IVF). There is no data in the literature regarding the issue in patients with mono-follicular growth. Direct aspiration or repeatedly flushing the follicle for those with a single follicle will be investigated.
Detailed description
Oocyte retrieval procedure This study aims to evaluate the effect of follicular flushing during oocyte retrieval procedure in patients with only one follicle. Double lumen needle will be used and direct follicular aspiration without flushing will be performed for all patients. If an oocyte will be obtained, the patient will be assigned to group A. If not, at least 1 course of follicular flushing will be used until retrieval of an oocyte and the patient will be assigned to group B.
Interventions
BEHAVIORALdirect follicular aspiration
direct follicular aspiration
BEHAVIORALfollicular flushing
follicular flushing
Sponsors
Centrum Clinic IVF Center
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* women aged 18-40 years * primary infertility * diminished ovarian reserve with single mature follicle on the day of oocyte retrieval
Exclusion criteria
* natural cycle IVF * presence of endometriosis/endometrioma(s) * Absence of cumulus oocyte complex following oocyte retrieval * Severe male factor infertility * Pre-implantation genetic screening cycles
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| mature oocyte | during oocyte retrieval procedure | Confirmation of cumulus oocyte complex (COC) after microscopic examination of follicular fluid by a senior embryologist for each patient. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| fertilization rate after micro-injection | 24 hours after microinjection procedure | Confirmation of pronuclei formation on the following day of microinjection by a senior embryologist in each patient. |
| clinical pregnancy rate per embryo transfer | untill 6 weeks | Confirmation of fetal cardiac activity on 5th or 6th week of gestation by a senior obstetrician in each pregnancy case. |
Countries
Turkey (Türkiye)
Outcome results
None listed