Lung Cancer (Diagnosis)
Conditions
Keywords
exosomes；lncRNA； lung cancer；
Brief summary
The study is to investigate the sensitivity and specificity of serum exosome noncoding RNA as a biomarker for the diagnosis of lung cancer
Detailed description
Conventional tumor markers for non-invasive diagnosis of Lung cancer (LC) exhibit insufficient sensitivity and specificity to facilitate detection of early lung cancer (ELC). The investigators aimed to identify ELC-specific exosomal lncRNA biomarkers that are highly sensitive and stable for the non-invasive diagnosis of ELC.Hence, in the present study, exosomes from the plasma of five healthy individuals and 30 LC patients and from culture media of four human bronchial epithelial cells and four cancer cells were isolated. Exosomal RNA profiling was performed using RNA sequencing to identify LC specific exosomal lncRNAs.
Interventions
DIAGNOSTIC_TESTcollect samples
collect serum samples and clinical features
Sponsors
Union Hospital, Tongji Medical College, Huazhong University of Science and Technology
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Subjects are willing to sign the informed consent form; * Normal subjects have no evidence of any disease; * Lung cancer patients are 18 to 75 years old, diagnosed as lung cancer by histopathology or cytopathology
Exclusion criteria
* People are unwilling to sign the informed consent form; * Patients with heart disease, rheumatic disease, allergic disease, COPD, pulmonary fibrosis, diabetes, thyroid disease, liver, kidney, brain disease and hematopoietic system disease; * Pregnant or lactating women; * Patients did not cooperate or participate in other clinical trials
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| The expression levels of serum exosome long non-coding RNA | the first day subjects are enrolled the outcome will be assessed；up to three years after the completion of the study |
| the expression levels of tumor biomarkers such as CEA, NSE, SCC, CYFR2A-1 | the first day subjects are enrolled the outcome will be assessed；up to three years after the completion of the study |
| the CT scans of the lung for the patients | the first day subjects are enrolled the outcome will be assessed；up to three years after the completion of the study |
Countries
China
Outcome results
None listed