Gut Microbiota, Metabolic Syndrome
Conditions
Keywords
Mango, Polyphenols, Inflammation, Insulin resistance, Transcriptomic, Metabolomic, Metagenomic
Brief summary
Mango is rich in bioactive compounds such as dietary polyphenols and carotenoids, which may explain its beneficial effect on health. Polyphenols accumulate in the gut where they can positively modulate the microbiota. As gut microbiota may have a strong influence on cardiometabolic health, we hypothesize that mango consumption improves metabolic profile in overweight or obese individuals through beneficial changes in gut microbiota. The study of metagenomics, transcriptomics and metabolomics will be used to validate this hypothesis.
Interventions
DIETARY_SUPPLEMENTMango consumption
Daily consumption of 280 g of frozen mangos during 8 weeks. The daily dose in equivalent to a 2-cups serving.
Sponsors
Laval University
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men and premenopausal women * At least one of the following : BMI between 25 and 40 kg/m2 or Waist circumference ≥ 80 cm for women and ≥ 94 cm for men * At least one of the following : TG ≥ 1.35 mmol/L or fasting insulinemia ≥ 42 pmol/L * Caucasian origin
Exclusion criteria
* Nicotine use * Metabolic disorders or use of medication that may affect parameters measured in the study * Use of dietary supplements or natural health product that may affect parameters measured in the study * Use of antibiotics in the last 3 months * Surgery in the last 3 months or planned during the study timeline * Alcohol consumption \>2 drinks/day * Weight change \>5% in the last 3 months * Mango aversion, allergy or intolerance * Dietary restrictions (vegetarian, gluten avoidance...) * Berries consumption \>1 serving/day * Pregnancy, breastfeeding or pregnancy planned within 3-6 months
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Changes in plasma insulin concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks |
| Changes in plasma glucose concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks |
| Changes in plasma triglycerides concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks |
| Changes in plasma total cholesterol concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks |
| Changes in plasma HDL cholesterol concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks |
| Changes in plasma LDL cholesterol concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in systolic blood pressure of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in diastolic blood pressure of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in insulin secretion of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in insulin sensitivity of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in glycated hemoglobin (HbA1c) in overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in plasma tumor necrosis factor (TNF-alpha) concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in waist circumference of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in plasma high-sensitivity C-reactive protein (hs-CRP) concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in gene expression in peripheral blood mononuclear cells (PBMC) of overweight/obese men and women taking frozen mangos daily | 8 weeks | Gene-level expression from \>20,000 annotated genes will be examined |
| Changes in plasma metabolites concentration in overweight/obese men and women taking frozen mangos daily | 8 weeks | Metabolites include acylcarnitines, glycerophospholipids, sphingolipids, sugars, amino acids, biogenic amines |
| Changes in gut microbiota composition in overweight/obese men and women taking frozen mangos daily | 8 weeks | Stool samples will be collected and whole metagenome shotgun sequencing analysis will be conducted |
| Changes in plasma lipopolysaccharide (LPS) concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in plasma LPS binding protein (LBP) concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in plasma interleukin-6 (IL-6) concentration of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in hip circumference of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in waist to hip circumference ratio of overweight/obese men and women taking frozen mangos daily | 8 weeks | Waist and hip circumference will be combined to report waist to hip ratio |
| Height of overweight/obese men and women taking frozen mangos daily | Baseline | — |
| Changes in weight of overweight/obese men and women taking frozen mangos daily | 8 weeks | — |
| Changes in body mass index (BMI) of overweight/obese men and women taking frozen mangos daily | 8 weeks | Weight and height will be combined to report BMI |
Countries
Canada
Outcome results
None listed