Assisted Reproduction, Polycystic Ovary Syndrome
Conditions
Brief summary
Ninety women with PCOS candidate for ICSI were randomized to 2 groups:Group A: fresh samples of PCOS patients undergoing IVF (no= 45) and Group B: frozen samples of PCOS patients undergoing IVF (no= 45). Morphologically good embryos will be cultured in a culture dish and the supernatants will be collected freshly from culture system at day 3 and stored at -20 ̊ c until being tested. Morphologically good embryos scheduled for freezing will be cryopreserved for less than one year and thawed, the supernatant will be collected and stored at -20 ̊ c until being tested. Quantification of mtDNA in fresh and frozen culture media using qPCR technique.The template DNA prepared and the dilution standards prepared will be used for qPCR to determine the amount of MtDNA and nuclear DNA in the sample using QuantiTect SYBR Green PCR Kit
Detailed description
Ninety women with PCOS candidate for ICSI were randomized to 2 groups:Group A: fresh samples of PCOS patients undergoing IVF (no= 45) and Group B: frozen samples of PCOS patients undergoing IVF (no= 45). Morphologically good embryos will be cultured in a culture dish and the supernatants will be collected freshly from culture system at day 3 and stored at -20 ̊ c until being tested. Morphologically good embryos scheduled for freezing will be cryopreserved for less than one year and thawed, the supernatant will be collected and stored at -20 ̊ c until being tested. Quantification of mtDNA in fresh and frozen culture media using qPCR technique.The template DNA prepared and the dilution standards prepared will be used for qPCR to determine the amount of MtDNA and nuclear DNA in the sample using QuantiTect SYBR Green PCR Kit (Qiagen)
Interventions
None listed
Sponsors
Cairo University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 38 Years
Healthy volunteers
No
Inclusion criteria
1. Age: 20-35 yrs. 2. BMI: ≤25. 3. PCO: diagnosed by Rotterdam criteria (at least two of the following): 1. Polycystic ovary morphology with one ovary being sufficient for diagnosis. 2. Hyperandrogenism: clinically or biochemically. 3. Anovulation or oligo-ovulation. 4. Gravidity and parity: Nulligravida. 5. Compliance: primary infertility
Exclusion criteria
* Known medical problems as diabetes mellitus, hypertension, cardiac, hepatic or renal problem. * Special habits of medical importance as smoking. * Other endocrinopathies
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| clinical pregnancy rate | 4 weeks after embryo transfer | detection of intrauterine gestational sac by transvaginal ultrasound |
Countries
Egypt
Outcome results
None listed