Infertility, Female
Conditions
Keywords
embryo quality, denudation, IVF, ICSI
Brief summary
The relation between the timing of COC denudation and oocyte /embryo quality is controversial. The aim of this study is to examine whether timing of denudation of sibling oocytes has any effect on embryo quality.
Detailed description
Retrieved sibling cumulus-oocyte complexes (COCs) will be randomly divided into two groups, in the first group denudation will be in 30 minutes after oocyte retrieval, in second group denudation will take place after 2 hours of incubation.
Interventions
OTHERearly denudation
COCs will be denudated in 30 min after oocyte retrieval
OTHERlate denudation
COCs will be denudated 2 hr after oocyte retrieval
Sponsors
Acıbadem Atunizade Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 40 Years
Healthy volunteers
No
Inclusion criteria
* COH with GnRH antagonist cycle * 5 or more oocytes collected
Exclusion criteria
* Fewer than 4 oocytes after retrieval * Previous history of total fertilization failure or oocyte maturation arrest * Male partner requiring surgical sperm retrieval (MESA or TESA).
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Number of good quality cleavage embryos | 3 days after microinjection | Quality of embryos will be assessed morphologically on day 3 |
| Embryo utilization rate | 1 week after microinjection | Embryo utilization rate is defined as the number of embryos utilized (transferred or cryopreserved) per number of 2PN zygotes. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Fertilization rate | 16-18 hour after microinjection | Proportion of injected oocytes with 2PN the day after injection |
| Cleavage rate | 2 days after microinjection | Proportion of cleaved zygotes on day 2 |
| Blastulation rate | 1 week after microinjection | Blastocyst rate per oocyte |
Countries
Turkey (Türkiye)
Outcome results
None listed