Age-related Changes in Sleep-wake Regulation

Age-related Changes in Sleep-wake Regulation: Effects of Sleep Loss on Possible Molecular Markers of Sleep Need

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT03813082

Enrollment

Registered

2019-01-23

Start date

2016-03-01

Completion date

2019-12-31

Last updated

2021-01-26

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Sleep Deprivation

Keywords

Positron emission tomography, Magnetic resonance spectroscopy, Aging

Brief summary

A sleep deprivation protocol combined with state-of-the-art, simultaneous positron emission tomography and magnetic resonance spectroscopy imaging will be employed to investigate the effects of sleep deprivation and aging on hypothesized molecular markers of sleep need.

Interventions

BEHAVIORALProlonged wakefulness

40 hours of prolonged wakefulness

Study design

Allocation

NON_RANDOMIZED

Intervention model

PARALLEL

Primary purpose

BASIC_SCIENCE

Masking

NONE

Eligibility

Sex/Gender

MALE

Age

20 Years to 70 Years

Healthy volunteers

Yes

Inclusion criteria

* healthy male individuals * age: 20-35 or 60-70 years * right-handed * non-smokers * moderate caffeine/alcohol consumption * normal BMI

Exclusion criteria

* Sleep/circadian rhythms disturbances * former brain injuries with loss of consciousness and brain anomalies * drug intake/consumption * medication intake * cardiac pacemaker * neurological disorders * prior participation (during past 2 years) in radiological or nuclear experiment

Design outcomes

Primary

Measure	Time frame	Description
Sleep-wake induced change in cerebral availability of mGluR5 (metabotropic glutamate receptors of subtype 5)	Change from baseline metabotropic glutamate receptors of subtype 5 availability after 33 hours of prolonged wakefulness and 8 hours of recovery sleep	Positron emission tomography

Secondary

Measure	Time frame	Description
Sleep-wake induced change in subjective state	Change from baseline sleepiness during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep	Subjective sleepiness as quantified with the Stanford Sleepiness Scale (range: 1 to 7)
Sleep-wake induced change in cognitive performance	Change from baseline cognitive performance during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep	Cognitive performance battery including tasks measuring sustained attention, executive functioning and working memory
Sleep-wake induced change in waking electroencephalogram (EEG)	Change from baseline waking EEG during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep	Spectral composition of the EEG in wakefulness
Sleep-wake induced change in potential molecular markers of sleep need	Change from baseline microRNA, fragile X mental retardation protein and brain-derived neurotrophic factor concentrations after 33 hours of prolonged wakefulness and 8 hours of recovery sleep	microRNAs, fragile X mental retardation protein, and brain-derived neurotrophic factor concentrations in peripheral blood
Sleep-wake induced change in sleep electroencephalogram (EEG)	Change from all-night baseline sleep EEG in 8 hours of recovery sleep after 33 hours of prolonged wakefulness	Spectral composition of the EEG during sleep
Sleep-wake induced change in glutamate concentration in prefrontal cortex and basal ganglia	Change from baseline glutamate concentration after 33 hours of prolonged wakefulness and 8 hours of recovery sleep	Magnetic resonance spectroscopy

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 21, 2026