Obesity, Childhood
Conditions
Keywords
Microbiome, Metabolome
Brief summary
In this study, the investigators aimed to observe changes in the gut microbial composition before and after the weight-loss intervention, including dietary control and exercise, in obese children. The main metabolites produced by gut microbiome, short chain fatty acids (SCFAs) will be also analysed. The investigators will analyse the change of gut microbiome composition and metabolites related with weight-loss intervention and the relationship between microbiome composition and metabolites.
Detailed description
Many recent studies have suggested that dysbiosis of the gut microbiome might related to obesity. There is growing evidence that the composition of gut microbiome changes in childhood obesity compared to normal-weight children, and it is attracting attention as an adjustable environmental factor in obesity treatment. It has been reported that gut microbiome could be changed due to environmental influences such as diet and exercise. In this study, the investigators aimed to observe changes in the gut microbial composition before and after the weight-loss intervention, including dietary control and exercise, in obese children. The main metabolites produced by gut microbiome, short chain fatty acids (SCFAs) would be also analysed to investigate the relationship with gut microbiome and with weight-loss intervention. Intervention is not performed for normal-weight children, and samples from normal-weight children will be used as a control for gut microbiome and biochemical tests.
Interventions
BEHAVIORALIndividualized Education Program
The every participants will have individualized education program about a way of dietary control and exercise in their usual life.
Sponsors
Hallym University Kangnam Sacred Heart Hospital
Study design
Allocation
NON_RANDOMIZED
Intervention model
FACTORIAL
Primary purpose
SUPPORTIVE_CARE
Masking
NONE
Intervention model description
Intervention arm: every obese children will have individualized education program about diet control and exercise in usual life. Control arm: normal weight children
Eligibility
Sex/Gender
ALL
Age
No minimum to 18 Years
Healthy volunteers
Yes
Inclusion criteria
* Obese children: Children ≥95 ‰ * Non-obese children: 5‰\<BMI \<85 ‰
Exclusion criteria
* Taking antibiotics, probiotics, or steroids for a month before visit * Taking probiotics-like products including yogurt for seven days before visit * Having enteritis symptoms including diarrhea for a month before visit * Chronic heart disease, chronic bowel disease, chronic liver disease, chronic kidney disease, endocrine disease, genetic diseases or congenital metabolic disorder
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in gut microbiome composition in stool samples | up to 3 months after enrollment | Bacterial diversity and composition using 16s rRNA sequencing. Especially Bacteroidetes and Firmicutes populations in fecal microbiome will be analyzed related with obesity. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Changes in short chain fatty acids of fecal samples | up to 12 months after enrollment | The main metabolites produced by gut microbiome, short chain fatty acids using gas chromatography- mass-spectrometry. |
Other
| Measure | Time frame | Description |
|---|---|---|
| The relationship between gut microbiome composition and fecal short chain fatty acid | up to 12 months after enrollment | The relationship between gut microbiome composition and metabolites will be analyzed by canonical correspondence analysis |
Countries
South Korea
Outcome results
None listed