Periodontal Diseases
Conditions
Brief summary
This current study aims to clarify the relationship between the occurrence of Entamoeba gingivalis infection and the specter of gingivitis & chronic periodontitis among Egyptian patients in comparison to healthy volunteers as a control group.
Detailed description
Three groups will be included in the current study. 40 Plaque samples will be collected from gingivitis patients (group 1), 40 from chronic periodontitis patients (group 2) plus 40 samples from healthy volunteers (group 3). Diagnosis of parasitic stages will rely on direct microscopic detection using permanent stains, Trichrome stain and haematoxylin and eosin stain in addition to direct wet mount examination.
Interventions
OTHERPlaque Samples
After carefully drying of the selected site and isolation by cotton roll, plaque samples will be collected by sweeping movement of the probe at gingival crevices of the tooth aspect with pervious inclusion criteria for obtaining of the plaque sample. Samples will be immediately dipped in sterile Eppendorf tubes containing PVA.
Sponsors
Cairo University
Study design
Observational model
CASE_CONTROL
Time perspective
OTHER
Eligibility
Sex/Gender
ALL
Age
35 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* Chronic Gingivitis patients with signs of clinical inflammation with no clinical attachment loss. * Chronic Periodontitis patients having probing depth of ≥3 mm and clinical attachment level ≥ 1 mm. * The control group: healthy subjects who attend the restorative dental clinic and had clinically healthy gingiva.
Exclusion criteria
* Patients with any systemic diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Presence of Entamoaba Gingivalis parasite in dental plaque assessed by direct microscopic examination | 2 weeks | Direct microscopic detection of Entamoaba Gingivalis in dental plaque samples using Trichrome stain |
Countries
Egypt
Outcome results
None listed