Exercise Physiology
Conditions
Brief summary
The purpose of this study is to determine the effect of age and fitness level on the physiological response to an acute bout of resistance or aerobic exercise.
Detailed description
Young and middle-aged to older adults who are physically active or not physically active will participate in two moderate to vigorous intensity exercise bouts: aerobic exercise and resistance training exercise. Blood will be collected to assess immune function and metabolism.
Interventions
randomized complete crossover for acute resistance training exercise session
OTHERAerobic training exercise
randomized complete crossover for acute aerobic exercise training exercise session
Sponsors
University of Houston
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
SUPPORTIVE_CARE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
* Young Adults: ages 18-30 * Older Adults: ages 55-75 and if a female postmenopausal * Participants classified as trained must participate in at least 30 minutes of moderate to vigorous intensity cardiovascular activity at least 3 days per week and engage in resistance training for all of the major muscle groups approximately 2 days / week. They must have upheld this activity for the past 3 months * Participants classified as untrained must be participating in less than 30 minutes of moderate to vigorous intensity cardiovascular activity and less than 2 days of resistance training per week for at least the last 3 months.
Exclusion criteria
* Have any contraindications to moderate to vigorous exercise * Range of motion restrictions that would prevent them from participating in aerobic or resistance training with proper form (they must be ambulatory) * aking medications (prescription or over the counter) known to influence immune function, including daily NSAID's, beta blockers, statins, bisphosphonates, or steroids. * Have known cardiovascular, respiratory, metabolic, or renal disease, with the exception of controlled hypertension (as defined by resting BP below 140/90) and/or controlled asthma (self-reported). * Be pregnant * Be unable to complete all visits (i.e. must not be planning to leave the Houston area long-term before concluding their participation in the study which will span several weeks) * Fall outside of a BMI range of (18.5 - 30) * Consume alcohol or recreational drugs for 24h prior to visits * Older women cannot be pre-menopausal (must have not had a menses for at least 12 months) * Unable to speak or read English * Participants must not have scheduling conflicts that would prevent them from reporting to the laboratory of integrated physiology 4 times over the course of the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Monocyte subset and function | Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise | Flow cytometry to classify monocyte subsets and in vitro LPS stimulation to assess monocyte function |
| NK cell function | Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise | Flow cytometry to identify NK cells and in vitro activity to assess NK activity |
| Circulating relative and absolute counts of T cell subsets | Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise | Flow cytometry to identify T cell subsets |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Serum cortisol | Pre-resistance training (RT) exercise, change from pre-RT exercise to immediately post-RT exercise, change from pre-RT exercise to 1hr post-RT exercise, change from immediately post-RT exercise to 1hr post-RT exercise | ELISA to measure circulating cortisol (serum) |
Countries
United States
Outcome results
None listed