Duchenne Muscular Dystrophy, Inflammatory Myopathy
Conditions
Keywords
Inflammatory myositis, Duchenne Muscular Dystrophy, Musculoskeletal ultrasound
Brief summary
The aim of our study is to Assess skeletal muscle structural status in children with inflammatory myositis and Duchenne muscular dystrophy using musculoskeletal ultrasound and to perform a longitudinal follow up of these changes over 2 years and to assess the relation between these findings with clinical parameters, functional scales, biochemical and electromyographic tests.
Detailed description
This study will be carried out on two groups: • Group (I): fifty children diagnosed to have duchenne muscular dystrophy and inflammatory myositis. Group (II): including 20 healthy children matching age and sex as control group. patients will be subjected to (A) Clinical evaluation 1. Complete history taking. 2. Thorough clinical examination. 3. Body mass index (BMI) assessment. 4. Quantitative muscle strength tests 5. Functional grading 6. Childhood Myositis Assessment Scale. 7 (B) Laboratory assessment: All patients will be subjected to the following measurements: 1. Serum creatine kinase levels (CK). 2. Serum Lactate dehydrogenase levels 3. Serum of Liver enzymes (SGOT& SGPT) levels. (C) Electromyographic (EMG) assessment: (D) Musculoskeletal ultrasound assessment (E) Statistical analysis
Interventions
DIAGNOSTIC_TESTQuantitative muscle ultrasound measurements
Quantitative ultrasound measurements will be performed to biceps, forearm flexors, quadriceps and tibialis anterior according to a standard protocol; for each muscle three consecutive measurements will be made to minimize variation in echo intensity during analysis .The captured images will be analyzed offline for echo intensity by means of computer-assisted grayscale histogram analysis.
Sponsors
Benha University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
2 Years to 16 Years
Inclusion criteria
* children with Duchenne muscular dystrophy (DMD). Diagnosis with DMD was established according to DMD diagnostic criteria (Jennekens et al., 1991). * children with juvenile dermatomyositis (JDM) according to Bohan and Peter diagnostic criteria ( (Bohan and Peter, 1975).
Exclusion criteria
* Patients with age less than 2 years were excluded from the study due to inability to perform manual muscle testing and functional scales. * If no final diagnosis could be established. * The presence of a concomitant illness that may result in peripheral neuropathy or myopathy.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Kendall's manual muscle testing | 24 months | Kendall's 0 -10 point scale measures strength of each muscle group score 0 is the weakest (worst) and 10 is the strongest (best). The following muscles were tested bilaterally: the biceps brachii muscle (BB), the forearm flexors (FF), the rectus femoris muscle (RF), the tibialis anterior muscle (TA) |
| Childhood myositis assessment scale | 24 months | used to assess the severity of muscle involvement in children with dermatomyositis. The scores for the 14 items are summated to give a total score ranging from 0 (worst) to 52 (best) |
| Serum creatine kinase (CK) levels | 24 months | CK measured in U/L using ELISA |
| Serum Lactate dehydrogenase (LDH) levels | 24 months | CK measured in IU/L using ELISA |
| Aspartate aminotransferase (AST) | 24 MONTHS | AST measured in U/L using ELISA |
| alanine aminotransferase (ALT) | 24 months | ALT measured in U/L using ELISA |
| motor unit potential (MUP) duration | 24 months | quantitative electromyography (QEMG) in the most affected rectus femoris and biceps brachii muscles will be performed and The motor unit potentials will be reviewed offline for the needle-detected EMG signals will be analyzed by the device software for the MUP duration measured in milliseconds. |
| motor unit peak-to-peak amplitude | 24 months | quantitative electromyography (QEMG) in the most affected rectus femoris and biceps brachii muscles will be performed and The motor unit potentials will be reviewed offline for the needle-detected EMG signals will be analyzed by the device software for the peak-to-peak amplitude measured in microvolt |
| motor unit area to amplitude ratio (AAR) | 24 months | quantitative electromyography (QEMG) in the most affected rectus femoris and biceps brachii muscles will be performed and The motor unit potentials will be reviewed offline for the needle-detected EMG signals will be analyzed by the device software for the motor unit AAR . |
Countries
Egypt
Outcome results
None listed