Anemia, Critical Illness, Lung Transplantation, Open Aortic Surgery
Conditions
Brief summary
The main goal of this pilot study is to assess the time course of eicosanoid profiles in intensive care unit (ICU) patients requiring packed red blood cell (PRBC) transfusion. Moreover we will analyze the change of levels of eicosanoids in patient plasma prior and after a PRBC transfusion as well as its correlation with levels of eicosanoids in the transfused PRBCs. These data will then be used to determine the estimated effect size necessary for the planning of future larger studies. We hypothesize that transfusion of PRBCs will modulate the eicosanoid profile in ICU patients. According to the Protocol filed with the Institutional Review Board of the Medical University of Vienna and patient's informed consent, subsequent sub analyses using samples of this study (e.g., determination of extracellular vesicles in PRBC samples and patient's plasma) will be performed.
Interventions
DIAGNOSTIC_TESTAnalysis of eicosanoid profile prior to and after PRBC transfusion
Using liquid chromatography-mass spectrometry to analyze the eicosanoid profile in patient plasma and aliquoted of the PRBCs prior to and after PRBC transfusion.
Sponsors
Medical University of Vienna
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 99 Years
Healthy volunteers
No
Exclusion criteria
* Age \<18 years or \>99 years * Pregnancy * \<12h since last PRBC transfusion * intake/administration of acetylsalicylic acid in groups 2 and 3 * intake/administration of cysteinyl leukotriene receptor antagonists (e.g. montelukast) * intake/administration of celecoxib, etoricoxib, parecoxib, ibuprofen, diclofenac, and naproxen in all groups * glucocorticoids given within 24h of transfusion in groups 1 and 3 * treatment with calcineurin inhibitors (e.g. tacrolimus) in groups 1 and 3 * treatment with alemtuzumab in groups 1 and 3 * administration of protamine for heparin reversal in groups 2 and 3
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Change of plasma eicosanoid concentrations in ICU patients subjected to PRBC Transfusion at any time Point compared to baseline | 60 minutes prior to 90 minutes after packed red blood cell transfusion |
Secondary
| Measure | Time frame |
|---|---|
| Changes of plasma eicosanoid profiles after PRBC transfusion compared to prior to PRBC transfusion in ICU patients | 60 minutes prior to 90 minutes after packed red blood cell transfusion |
| Correlation of plasma eicosanoid profiles in the transfused PRBCs and the change of their plasma levels in ICU patients | 60 minutes prior to 90 minutes after packed red blood cell transfusion |
Countries
Austria
Outcome results
None listed