Infertility
Conditions
Brief summary
The pH stability during human gamete handling for intracytoplasmic sperm injection (ICSI) is physiologically sound. As the pH change for bicarbonate-buffered medium, scientists assumed whether the use of HEPES or MOPS buffered medium would provide more stable pH, thereby better outcomes. However, the oil use and time frame outside the incubators have not been accounted for. This study is to evaluate the use of HEPES buffered medium compared with the bicarbonate-buffered medium on ICSI outcomes.
Interventions
OTHERBicarbonate-buffered handling medium
The investigators will evaluate whether the usual bicarbonate culture medium can replace the HEPES buffered medium effectively.
Sponsors
Banoon IVF Center
Quena IVF Center
Muhammad Fawzy
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
No
Inclusion criteria
* ICSI indicated cycles
Exclusion criteria
* severe medical illness
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| live birth rate | 40 weeks | viable neonate after 30 weeks of Gestation |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Clinical pregnancy rate | 12 weeks | a positive heartbeat on ultrasound at ≥ 7 weeks of gestation |
| biochemical pregnancy rate | two weeks | positive b-hCG at ≥ 14 days following embryo transfer |
| embryo developmental rates | 6 days of culture | fertilized oocyte per injected ones and developed embryos per fertilized oocytes |
| Ongoing pregnancy rate | 16 weeks | Continued pregnancy after week 12 of gestation |
Countries
Egypt
Outcome results
None listed