Hypertension,Essential
Conditions
Keywords
Pannexin-1 channel
Brief summary
Sympathetic nervous activity plays an important role in the development of hypertension and studies have shown that the pannexin-1 channel is involved in the signalling of the sympathetic activity to the vascular bed. The main project aim is to investigate the effects of 10 weeks of high intensity training on the effect of the sympathetic nervous activity on vascular function in individuals with essential hypertension. A secondary aim is to assess the role of essential hypertension and physical activity on vascular endothelial function,
Interventions
OTHERTraining
10 weeks of high intensity exercise training. 2-3 trainings per week of approx. 40 min.
Sponsors
Lundbeck Foundation
Danish Council for Independent Research
University of Copenhagen
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Caregiver, Investigator)
Intervention model description
Randomized double blinded case-control interventional study
Eligibility
Sex/Gender
ALL
Age
35 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Sedentary men and women age 35 - 65 years * Resting blood pressure \>140/90 mmHg or Resting blood pressure \<140/90 mmHg * Essential hypertension * Non or exclusively antihypertensive medication
Exclusion criteria
* Hypertensive condition other than essential hypertension * Other chronical diseases * Smoking * Excess alcohol intake or drug abuse
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blood pressure | Before and after 8 weeks of training | Changes in systolic and diastolic blood pressure |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Pannexin channel distribution in skeletal muscle tissue | Before and after 8 weeks of training | Pannexin channel distribution is assessed immunohistochemically in muscle samples |
| Sympathetic activity | Before and after 8 weeks of training | Direct measurement of Muscle Sympathetic Nervous Activity via microneurography |
| Vascular function | Before and after 8 weeks of training | Vascular conductance measured by ultrasound doppler during infusion of vasoactive substances |
| Mitochondrial function in endothelial cells | Before and after 8 weeks of training | Endothelial cell mitochondrial respiration is measured by High Resolution Respirometry |
| Reactive oxygen species formation in in endothelial cells | Before and after 8 weeks of training | Endothelial cell production of reactive oxygen species is measured by High Resolution Respirometry |
| Pannexin channel function assessed in vivo | Before and after 8 weeks of training | Pannexin channel function is assessed by measurement of vascular conductance with and without channel inhibition |
| Platelet reactivity | Before and after 8 weeks of training | Platelet reactivity is assessed by multiplate aggregometry |
| Maximal oxygen uptake | Before and after 8 weeks of training | Direct Measurement of oxygen uptake during a graded test on cycle ergometer |
| Body Composition | Before and after 8 weeks of training | Measured by Dual energy x-ray absorptiometry |
| Endothelial cell characterization | Before and after 8 weeks of training | Assessment of protein and mRNA-levels of proteins of relevance for endothelial function |
| Skeletal muscle characterization | Before and after 8 weeks of training | Assessment of protein and mRNA-levels of proteins of relevance for skeletal muscle function |
| Rheology | Before and after 8 weeks of training | Platelet rheology. The viscoelastic measurements are based on attainment of the Gel Point (GP) from which the fractal dimension, df, is determined. |
Countries
Denmark
Outcome results
None listed