Safety and Efficacy of Vaginal Microbiota Transplant (VMT) in Women With Bacterial Vaginosis (BV)

Inclusion criteria

Inclusion criteria that apply to potential Donor participants: 1. Capable of reading and writing English and voluntarily provide written informed consent to participate in the study and comply with all study procedures. 2. Generally healthy, pre-menopausal women age 18-45 years old. 3. Regular predictable menstrual cycles or amenorrheic for at least 3 months due to use of long-acting progestin or continuous use of oral contraceptives. 4. Free of vaginal symptoms, such as odor, discharge, or itching. 5. Willing to be asked questions about personal medical, sexual, and behavioral history. 6. Willing to self-collect cervicovaginal secretions and vaginal swab samples. 7. Agree to abstain from using insertive vaginal feminine products (i.e. Tampons, menstrual cups, sex toys), other vaginal products (cleansing products, spermicides, lubricants, hygiene powders and sprays), vaginal and anal intercourse, taking baths, going swimming, sitting in hot tub, or wearing thong underwear during the entire sample donation period (from screening to the final donation). Sanitary napkins are acceptable. Inclusion criteria that apply to potential Recipient participants: 1. Capable of reading and writing English and voluntarily provide written informed consent to participate in the study and comply with all study procedures. 2. Generally healthy, pre-menopausal women age 18-45 years old. 3. Experiences recurrent bacterial vaginosis (BV) (at least 3 diagnoses in their lifetime), and has received treatment for BV on at least 1 prior occasion in the past 5 years. 4. Currently needing treatment for vaginal symptoms clinically diagnosed as BV (\>= 3 Amsel criteria and confirmed in the laboratory by Nugent score \>= 4) and suitable for 0.75% metronidazole vaginal antibiotic treatment. 5. Regular predictable menstrual cycles or amenorrheic for at least 3 months due to use of long-acting progestin or continuous use of oral contraceptives. 6. Women who are not sterilized or who are not using an Intra-uterine Device (IUD) or form of hormonal contraception must agree to barrier contraception (i.e. Condoms, diaphragm) for heterosexual intercourse during the study. Counseling will be provided for use of barrier contraception without the use of adjunctive spermicide or lubricant. 7. Willing to be asked questions about personal medical, sexual, and behavioral history. 8. Willing to self-collect cervicovaginal secretions and vaginal swab samples. 9. Willing to undergo VMT procedure. 10. Agree to abstain from vaginal intercourse, unless using a condom, for at least the 28 days after the VMT procedure. Condoms will be provided. 11. Agree to avoid taking baths, swimming, sitting in a hot tub, or wearing thong underwear for at least the 28 days after the VMT procedure. 12. Agree to abstain from using insertive vaginal feminine products (i.e. Tampons, menstrual cups, sex toys), vaginal cleansing products, spermicides, lubricants, or other vaginal products not approved by the study investigators after the VMT procedure through visit 8 (\ 6 months). Sanitary napkins are acceptable.