Estimation of Level of ERAP1 Expression in Lesional and Non Lesional Skin of Psoraitic Patients and Healthy Controls.

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT03767114

Enrollment

Registered

2018-12-06

Start date

2017-01-31

Completion date

2018-12-31

Last updated

2018-12-06

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Psoriasis

Brief summary

A 4mm Punch biopsy is obtained from lesional and non lesional skin of 25 psoraitic patients as well as of 30 controls. Skin biopsy from patients (psoriasis lesional and non lesional) & control will be kept in lysis solution (which contains RNAase inhibitor) for the stability of the parameter to be assessed. ERAP-1 gene expression will Research Template 5 Final Version: 8/3/2 016 be detected by quantitative real time -PCR, which include RNA extraction, cDNA synthesis & PCR.

Interventions

DIAGNOSTIC_TESTRT-PCR for ERAP1 detection

Reverse transcriptase -Polymerase Chain Reaction for ERAP1 detection

Study design

Allocation

RANDOMIZED

Intervention model

SINGLE_GROUP

Primary purpose

DIAGNOSTIC

Masking

DOUBLE (Subject, Investigator)

Eligibility

Sex/Gender

ALL

Age

18 Years to 75 Years

Healthy volunteers

Yes

Inclusion criteria

* patients with psoriasis vulgaris * patients over 18 years of age * both sexes

Exclusion criteria

* patients on treatment for psoriasis within the last two months * assoiciated autoimmune diseases eg SLE * pregnancy

Design outcomes

Primary

Measure	Time frame	Description
Estimation of lesional ERAP1 in psoriatic skin	Maximum 2 years	DPCRetection of level of expression of ERAP 1 in lesional skin of psoriasis patients by RT-

Secondary

Measure	Time frame	Description
Estimation of non lesional ERAP1 in psoriatic skin	Maximum 2 years	Detection of level of expression of ERAP 1 in non lesional skin of psoriasis patients by RT-PCR.

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026