Dehydration (Physiology), Glucose Metabolism Disorders, Water Deprivation
Conditions
Keywords
water, vasopressin
Brief summary
There is a growing body of evidence suggesting low water intake and elevated levels of the hormone vasopressin exacerbate glucose regulation. This project will examine the physiological mechanism by which low water intake impairs glucose homeostasis. In the current proposal we aim to: 1) quantify the degree of glucose impairment as a response to elevated vasopressin due to low water intake and 2) identify the physiological mechanism by which elevated vasopressin, as a response to low water intake, impairs glucose regulation
Interventions
OTHERWater intake manipulation
for 5 days prior to each experiment subjects will undergo manipulation of their water intake.
Sponsors
Arizona State University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
30 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
BMI 27.5-35
Exclusion criteria
* BMI \<27.5 or \>35 * high physical activity * diabetes * impaired liver or kidney function * cardiovascular disease * weight change of more than 3 kg in the past month * pregnancy * previous surgery on digestive tract (except appendectomy) * fluid balance will be exclusionary such as * serotonin re-uptake inhibitors.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| amount of glucose metabolized M | 220-240 min & 340-360 min of the protocol | amount of glucose metabolized during the euglycemic & Hyperglycemic clamp |
| metabolic clearance rate for insulin | 220-240 min & 340-360 min of the protocol | metabolic clearance rate for insulin during the euglycemic & Hyperglycemic clamp |
| quantity of glucose metabolized per unit plasma insulin concentration | 220-240 min & 340-360 min of the protocol | quantity of glucose metabolized per unit plasma insulin concentration during the euglycemic & Hyperglycemic clamp |
| Rate of glucose appearance & disappearance | 220-240 min & 340-360 min of the protocol | Rate of glucose appearance & disappearance during the euglycemic & Hyperglycemic clamp |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| glucagon | 0 min, 240 min & 360 min of the protocol | glucagon in response to water manipulation |
| Cortisol | 0 min, 240 min & 360 min of the protocol | Cortisol in response to water manipulation |
| Plasma copeptin | 0 min, 240 min & 360 min of the protocol | Plasma copeptin in response to water manipulation |
Outcome results
None listed