Healthy
Conditions
Keywords
meal ingestion, metabolomic responses, hedonic sensations, homeostatic sensations
Brief summary
Aim: to determine the effect of gender on the sensory and metabolomic responses to a standard probe meal. Participants (12 men and 12 women) will be instructed to eat a standard dinner the day before, to consume a standard breakfast at home after overnight fast, and to report to the laboratory, where the test meal will be administered 4 h after breakfast. Studies will be conducted in a quiet, isolated room with participants sitting on a chair. Participants will ingest a standard probe meal (750 Kcal). Perception of homeostatic sensations (hunger/satiation, fullness) and hedonic sensations (digestive well-being, mood) will be measured at 5 min intervals 10 min before and 20 min after ingestion and at 10 min intervals up to 60 min after the probe meal. Heart rate variability, blood pressure and body temperature were measured before and after the meal. Blood samples for metabolomic analysis were taken before and 30 min after the meal.
Interventions
OTHERProbe meal
The probe meal (total caloric content 750 Kcal) will be ingested at a standard rate in 10 min.
Sponsors
Hospital Universitari Vall d'Hebron Research Institute
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Non obese
Exclusion criteria
* history of gastrointestinal symptoms * prior obesity * use of medications * history of anosmia and ageusia * current dieting * alcohol abuse * psychological disorders * eating disorders
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fullness sensation induced by the probe meal | 1 day | Gender differences in fullness sensation \[(measured by a 10 cm scale graded from 0 (not at all) to 10 (very much)\] by repeated measures ANCOVA (dependent variable: postprandial sensations scores; between and within subject's factors: gender and time, respectively; covariate: premeal scores). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in circulating metabolites induced by the probe meal | 1 day | Change in low-molecular weight metabolites and lipoproteins analysed using nuclear magnetic resonance. |
| Change in hunger/satiety induced by the probe meal | 1 day | Gender differences in hunger/satiety \[(measured by a 10 cm scale graded from -5 (extremely hungry) to +5 (completely satiate)\] by repeated measures ANCOVA (dependent variable: postprandial sensations scores; between and within subject's factors: gender and time, respectively; covariate: premeal scores). |
| Change in digestive well-being induced by the probe meal | 1 day | Gender differences in digestive well-being \[(measured by a 10 cm scale graded from -5 (extremely unpleasant sensation) to +5 (extremely pleasant sensation)\] by repeated measures ANCOVA (dependent variable: postprandial sensations scores; between and within subject's factors: gender and time, respectively; covariate: premeal scores). |
| Change in mood induced by the probe meal | 1 day | Gender differences in mood \[(measured by a 10 cm scale graded from -5 (negative) to +5 (postive)\] by repeated measures ANCOVA (dependent variable: postprandial sensations scores; between and within subject's factors: gender and time, respectively; covariate: premeal scores). |
Countries
Spain
Outcome results
None listed