Lipids and Other Bioactive Compounds of Human Breast Milk

Lipids and Other Bioactive Compounds of Human Breast Milk and Infant Formulas.

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT03746886

Enrollment

Registered

2018-11-20

Start date

2018-11-01

Completion date

2022-05-24

Last updated

2026-05-07

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Breast Milk Collection

Keywords

breast milk, lipids, bioactive compounds, infant formula

Brief summary

The biochemical differences in the composition of breast milk and formula have not been profoundly established in all compound groups. Increased understanding of breast milk composition, the normal variation of various bioactive compounds and their levels is required to be able to develop formulas better corresponding the breast milk, and ensure the optimal development of also the formula-fed babies.

Interventions

OTHERBreast-milk

A single breast-milk sample will be collected from 20 women. Milk samples will be pooled for analysis.

Study design

Observational model

CASE_ONLY

Time perspective

OTHER

Eligibility

Sex/Gender

FEMALE

Age

18 Years to 45 Years

Inclusion criteria

* breast-fed child was born full-time and is healthy. * age of the breast-fed child is 0-6 months

Exclusion criteria

* breast-fed child was born prematurely (gestation week \<37)

Design outcomes

Primary

Measure	Time frame	Description
Relative proportions (%) of regioisomers within selected molecular species (acyl carbon number:number of double bonds 50:1 and 52:2) in triacylglycerols.	11/2018-12/2019	Isolation of lipids from the milk with solvent extraction. Isolation of triacylglycerols with solid phase extraction. Negative ionization of the triacylglycerols with ammonia. Selection of the molecular weight species in the first quadruple of the MS/MS. Fragmentation of the selected ions with argon. Separation of the fragment ions in the second quadrupole of the MS/MS. Calculation of the relative proportions of regioisomers with a computer software.
Relative proportions (%) of fatty acids in triacylglycerols of human milk.	11/2018-12/2019	Isolation of lipids from the milk with solvent extraction. Isolation of triacylglycerols with solid phase extraction. Quantitation with gas chromatograph coupled with flame ionization detector in relation to external standards.
Quantity (mg/ml) of lipid soluble compounds in triacylglycerols of human milk.	11/2018-12/2019	Isolation of lipids from the milk with solvent extraction. Gravimetric analysis of the lipids.

Countries

Finland

Contacts

PRINCIPAL_INVESTIGATORKaisa M Linderborg, PhD

University of Turku

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: May 8, 2026