Fatty Liver
Conditions
Brief summary
The purpose of this study is to examine whether a low carbohydrate diet as compared to simple calorie restriction decreases hepatic lipid content and changes hepatic mitochondrial flux in humans.
Detailed description
In this study, the investigators will examine whether a low carbohydrate diet as compared to simple calorie restriction will change hepatic lipid content and mitochondrial flux in humans. Before and after a low carbohydrate diet and a simple calorie restriction intervention, rates of hepatic mitochondrial flux will be assessed using positional isotopomer analysis of 3-13C-lactate during a 3 hour basal period. Rates of whole body ketogenesis will be determined using \[13C4\]β-hydroxybutyrate, and whole body glucose metabolism will be measured using \[2H7\]glucose. Body composition will be determined with bioelectrical impedance; hepatic lipid content will be measured with MRS. Whole-body oxidation of lipids, carbohydrates and protein will be determined using indirect calorimetry.
Interventions
BEHAVIORALLow Carbohydrate Diet
Ketogenic dietary intervention
BEHAVIORALSimple Calorie Restriction Diet
Simple calorie restriction dietary intervention
Sponsors
Helsinki University Central Hospital
Study design
Allocation
NON_RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Low Carbohydrate Diet versus Simple Calorie Restriction Diet
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Age 18-70 * Subjects must be able to communicate meaningfully with the investigator and must be legally competent to provide written informed consent
Exclusion criteria
* chronic liver disease other than NAFLD * chronic disease associated with hepatic steatosis such as diabetes mellitus * use of drugs known to affect hepatic steatosis * contraindications for MRI * pregnancy or nursing at the time of the study
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in hepatic triglyceride content | 1 week | Change in hepatic triglyceride content will be measured using 1H magnetic resonance spectroscopy (MRS). |
| Change in hepatic mitochondrial flux | 1 week | Change in the rate of hepatic mitochondrial flux (Vcs) will be assessed using 3-13C-lactate infusion. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in beta-hydroxybutyrate production rate | 1 week | Change in the rate of beta-hydroxybutyrate production will be assessed using \[13C4\]β-hydroxybutyrate infusion. |
| Change in glucose production rate | 1 week | Change in the rate of glucose production will be assessed using \[2H7\]glucose infusion. |
Countries
Finland
Outcome results
None listed