Chronic Hepatitis b
Conditions
Keywords
chronic hepatitis B, regulating B cells, T help 17 cells, Interleukin 35
Brief summary
The investigators observed the level of IL-35 secreting B regulatory (IL-35+Bregs) cells in peripheral blood cells in patients with chronic hepatitis B, and analysed the relationship between the IL-35+Bregs level and disease stage, and Th1 and Th2 cells level.
Detailed description
Regulatory B cells (Bregs) are involved in the immune tolerance process through multiple pathways and Bregs are involved in the process of chronic HBV infection. Here the investigators intended to investigate the regulating role and the mechanism of CD19+ IL-35+ cells on host T cell immune during chronic HBV infection by using in vitro cell sorting, B cell differentiation induction, Transwell cell co-culture system and other methods.
Interventions
None listed
Sponsors
Changhai Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
17 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
* Clinical diagnosis of chronic hepatitis B virus infection * 17\<age\<70 * Can read, understand and sign patients informed consent
Exclusion criteria
* Co-infection with HCV,HIV,HDV etc. * Have malignancy * Have uncontrollable extra-hepatic disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| IL-35+Bregs level | ENROLLED DAY | IL-35 secreting regulatory B cells level in patients with CHB |
| IL-35+Bregs regulatory effect | enrolled day | regulatory effect of IL-35+Bregs on T cell immune |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| IL-35+Bregs phenotype | enrolled day | the main phenotype of IL-35 secreting Bregs |
Countries
China
Outcome results
None listed