Fertility, Spermatic Parameters
Conditions
Keywords
Cryo preservation, Spermatic nuclear quality
Brief summary
The reference technique for the conservation of gametes is storage in liquid nitrogen but new vats of nitrogen vapor (storage over liquid nitrogen) or in dry phase (storage in an insulated compartment of liquid nitrogen in a tank Liquid nitrogen) also allow the storage of flakes. The purpose of this work is to evaluate the dry-phase cryopreservation technique of liquid nitrogen compared with liquid-phase storage, depending on the duration of cryopreservation.
Detailed description
Objective: To evaluate the effects of cryopreserved sperm in dry and liquid phase nitrogen at 3 and 6 month on sperm numeration, motility, vitality, morphology, acrosomal integrity and DNA fragmentation. Design: Experimental study, investigator was blinded to the type of Cryopreservation. Patient(s): Semen samples were collected from patients who came in laboratory for semen analysis Intervention: Samples were frozen with a programmable freezing unit. Each semen sample was divided into two aliquots. One aliquot was plunged into liquid nitrogen and the other was stored in dry-phase nitrogen for 3 or 6 month. Thawing was performed at room temperature.
Interventions
OTHERcryopreservation
Samples were frozen with a programmable freezing unit. Each semen sample was divided into two aliquots. One aliquot was plunged into liquid nitrogen and the other was stored in dry-phase nitrogen for 3 or 6 month. Thawing was performed at room temperature
Sponsors
University Hospital, Clermont-Ferrand
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
MALE
Age
18 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Men undergoing routine semen analysis for infertility for reproductive medicine of university hospital in Clermont-Ferrand
Exclusion criteria
* women
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Sperm DNA integrity | at 3 months | the spermatic DNA integrity can be quantified by TUNEL method. The result will be expressed as a percentage of fragmented DNA |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Sperm parameter post cryopreservation | at 3 and 6 months | The Sperm motility will be measured according to WHO recommendations |
| Lab parameter post cryopreservation | at 3 and 6 months | The Sperm motility will be measured according to WHO recommendations |
| Spermatic DNA Compaction | at 3 and 6 months | The spermatic DNA Compaction will be determinated by chromomycine A3 labelling (CMA3). A sperm is good when it has at least 30% of positive spermatozoa to CMA3 assay |
| Acrosomal integrity | at 3 and 6 months | The acrosomal integrity will be determinated by PSA-FITC labelling. |
Countries
France
Outcome results
None listed