Rabies
Conditions
Keywords
rabies vaccination
Brief summary
To study immunological tolerance effect after frequent rabies booster vaccination
Detailed description
Previous studies had shown lower antibody titers among those who received frequent rabies booster vaccination, compared to ones who had got primary immunization. We study immunological cells i.e., regulatory T cell, regulatory B cell, T follicular helper cell, and cytokines in those who received frequent rabies booster vaccination.
Interventions
BIOLOGICALBlood drawn after rabies vaccine booster vaccination
All participants would be given one dose of rabies vaccine and monitor the immunological cells i.e., regulatory T cell, regulatory B cell, T follicular helper cells, cytokines i.e., IL-10, TGF-Beta, and Rabies neutralizing antibody titers, before and after the booster injection
Sponsors
Queen Saovabha Memorial Institute
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
SINGLE (Outcomes Assessor)
Masking description
The serum samples from all participants would be labelled as the codes, of which the laboratory technicians could not access to the groups or other details of the participants.
Intervention model description
There would be two study groups. Those who had received primary immunization would be the control group. While persons who had received primary immunization and later received booster vaccination for at least 3 times would be the study group. All participants would be given one dose of rabies vaccine and monitored the immunological cells and cytokines before and after the rabies booster injection.
Eligibility
Sex/Gender
ALL
Age
20 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy adults * Previously received rabies vaccination
Exclusion criteria
* Had immunocompromised conditions * Received blood or blood product within 3 months * Received anti-malarial drugs
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Rabies specific regulatory T cells | Change from baseline of numbers of Rabies specific regulatory T cells before (baseline) and one month after receipt of rabies booster vaccination were compared between 2 arms | Rabies specific regulatory T cells are measured by flow cytometry method |
| Rabies specific regulatory B cells | Change from baseline of numbers of Rabies specific regulatory B cells before (baseline) and one month after receipt of rabies booster vaccination were compared between 2 arms | Rabies specific regulatory B cells are measured by flow cytometry method |
| Rabies specific T follicular helper cells | Change from baseline of numbers of Rabies specific T follicular helper cells before (baseline) and one month after receipt of rabies booster vaccination were compared between 2 arms | Rabies specific T follicular helper cells are measured by flow cytometry method |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Rabies Neutralizing Antibody Titers (RNab) | before (baseline) and one month after receipt of rabies booster vaccination | Rabies Neutralizing Antibody Titers would be represented and compared by geometric mean titers (GMTs) |
Other
| Measure | Time frame | Description |
|---|---|---|
| Cytokines | Change from baseline of quantitative measurement of cytokines levels would be measured before (baseline) and one month after receipt of rabies booster vaccination and compared between 2 arms | Cytokines i.e., IL-10, TGF-Beta are measured by ELISA |
Countries
Thailand
Contacts
Primary ContactSuda Sibunruang, MD
Backup ContactTerapong Tantawichien, MD
Outcome results
None listed