Mitochondrial Biogenesis
Conditions
Keywords
Acute Exercise, Mitochondria, Skeletal Muscle, PGC1α, Mitochondrial Biogenesis, Intracellular Signalling
Brief summary
This crossover study investigates the effects of restricting arterial blood flow during cycling exercise on PGC-1α and its upstream signalling pathways. In a randomized order, participants will exercise once for 30 minutes with restricted blood flow and then will exercise once for 30 minutes with no blood flow restriction.
Interventions
OTHERBFR Exercise
Participants will exercise on a recumbent bike placed on a 45 degree incline (BFR).
OTHERCTL Exercise
Participants will exercise on a recumbent bike placed on flat ground (CTL).
Sponsors
Queen's University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Investigator, Outcomes Assessor)
Masking description
Allocation concealment, assessor blinding
Eligibility
Sex/Gender
MALE
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* 3 hours of physical activity per week * No previous cycling training * No concurrent involvement in another training program * Body mass index \< 30 kg/m2
Exclusion criteria
* Presence of cardiovascular disease * Presence of metabolic disease * Taking regular oral medication * Current smoker
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) messenger ribonucleic acid (mRNA) | 15 minutes before exercise and 3 hours post-exercise for both exercise sessions | Fold-change in PGC-1α mRNA expression |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Total and phosphorylated p38 mitogen-activated protein kinase (MAPK) | 15 minutes before exercise and immediately post-exercise for both exercise sessions | Fold change in p38 MAPK phosphorylation |
| Total and phosphorylated acetyl coenzyme A carboxylase (ACC) | 15 minutes before exercise and immediately post-exercise for both exercise sessions | Fold change in ACC phosphorylation |
| Total and phosphorylated protein kinase A (PKA) | 15 minutes before exercise and immediately post-exercise for both exercise sessions | Fold change in PKA phosphorylation |
| Acetylated p53 | 15 minutes before exercise and immediately post-exercise for both exercise sessions | Fold change in acetylated p53 |
| Catecholamine concentrations | 15 minutes before exercise and immediately post-exercise for both exercise sessions | Catecholamine concentrations in venous blood |
| Heart rate (HR) | During both exercise sessions | Average HR between conditions |
| Revolutions per minute (RPM) | During both exercise sessions | Average RPM between conditions |
| Ratings of perceived exertion (RPE) | During both exercise sessions | Average RPE between conditions |
| Oxygenated and deoxygenated hemoglobin | During both exercise sessions and up to 10 minutes after (for cuff occlusion) | Average oxygenated and deoxygenated hemoglobin as a percentage of same day max values |
| Blood lactate | During both exercise sessions (lactate); 15 minutes before and immediately after both exercise sessions | Average \[lactate\] between conditions |
Countries
Canada
Outcome results
None listed