Diabetes, Heart Failure
Conditions
Keywords
GLP-1, Neprilysin, DPP-4
Brief summary
In the current study we wish to investigate the effects on glucagon-like peptide-1 (GLP-1) of a neprilysin inhibitor
Detailed description
Glucagon-like peptide-1(GLP-1) is secreted during a meal and increases glucose induced insulin secretion. The enzyme dipeptidyl peptidase 4(DPP-4) cleaves intact GLP-1 within minutes and DPP-4 inhibitors are therefore used for treatment of diabetic hyperglycemia. A few animal studies have implicated the enzyme neutral endopeptidase 24.11 (24.11) in the degradation of GLP-1 but if this is the case in humans is unknown. We therefore administered a NEP inhibitor, 194mg sacubitril, a DPP-4 inhibitor (sitagliptin 100mg the night before and 100mg 2 hours before), both, or place, to nine healthy men during a standardized meal and measured plasma concentrations of GLP-1
Interventions
DRUGEntresto
Single dose administration of Entrestro 194 mg sacubitril / 206 mg valstartan
2 x 100mg sitagliptin as single dose.
DRUGPlacebos
No treatment
Sponsors
Nicolai Jacob Wewer Albrechtsen
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
TREATMENT
Masking
NONE
Intervention model description
The participants are on 4 study days with wash out period of minimum 2 weeks randomized to 4 intervention arms
Eligibility
Sex/Gender
MALE
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* men * body mass index between 20-25
Exclusion criteria
* acute diseases within the two weeks * chronic diseases * smoker * alcoholism, drug addiction or recent weight loss * blood donation within the last 3 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| GLP-1 | 3 hours after treatment ( during the subsequent standardized meal) | changes in plasma GLP-1 using immunological methods: intact and total GLP-1 |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| C-peptide | 3 hours after treatment ( during the subsequent standardized meal) | changes in C-peptide using immunological methods |
| Glucagon | 3 hours after treatment ( during the subsequent standardized meal) | Changes in Plasma glucagon concentrations using different analytical methodologies |
Other
| Measure | Time frame | Description |
|---|---|---|
| Blood glucose | 3 hours after treatment and during the subsequent standardized meal | changes in blood glucose using immunological methods |
| GIP | 3 hours after treatment ( during the subsequent standardized meal) | changes in plasma GIP using immunological methods |
Countries
Denmark
Outcome results
None listed