Osteoarthritis
Conditions
Keywords
Osteoarthritis, Crucumin, Flowcytometry
Brief summary
In this study, the effects of crucumin on cellular and humoral immune system in patients with osteoarthritis will be investigated. Concentration of CXCL8, April, CX3CL1 and IL-17 will be evaluated with ELISA. TH-1, TH-17, TReg and Ly.B cells count will be measured by Flowcytometry. MicroRNA-720, MicroRNA-155, MicroRNA-16 and MicroRNA-146a gene expression will be measured with Real Time PCR technique.
Detailed description
The purpose of this study was to evaluate the effect of the active ingredient of crucumin on the reduction of symptoms of osteoarthritis. Of the 30 patients participating in this study, a blood sample will be taken at the beginning of the study, and then after three months of taking the crucumin. To evaluate the response to treatment, after each person sampling, we will analyze cell count for Th-1, Th-17, TReg and Ly.B by flowcytomtry method. Also we will measure cytokines level of IL-17, CXCL8, CX3CL1 and April by ELISA method. Finally we evaluate the gene expression of MicroRNA-720, MicroRNA-155, MicroRNA-146a and MicroRNA-16 by Real Time PCR technique.
Interventions
DRUGCrucumin
Evaluation of the effect of Crucumin in patients with osteoarthritis
DRUGPlacebo
Placebo
Sponsors
Mashhad University of Medical Sciences
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
Osteoarthritis patients who have been diagnosed with a rheumatologist physician based on clinical examinations and laboratory tests
Exclusion criteria
Affected by any other acute or chronic underlying disease
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| IL-17 | Baseline | Cytokine level |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| TH-1 | Baseline and three months | Cell counting |
| MicroRNA-720 | Baseline and three months | Gene expression |
| CXCL8 | Baseline and three months | Cytokine level |
| CXCL1 | Baseline and three months | Cytokine level |
| April | Baseline and three months | Cytokine level |
| IL-17 | Baseline and three months | Cytokine level |
| TReg | Baseline and three months | Cell counting |
| Ly.B | Baseline and three months | Cell counting |
| MicroRNA-155 | Baseline and three months | Gene expression |
| MicroRNA-146a | Baseline and three months | Gene expression |
| MicroRNA-16 | Baseline and three months | Gene expression |
| TH-17 | Baseline and three months | Cell counting |
Countries
Iran
Contacts
Primary ContactMojgan Mohammadi, Ph.D
Backup ContactMahdi Atabaki, Student
Outcome results
None listed