Obesity, Pre Diabetes
Conditions
Brief summary
Based on our hypothesis that orally administered resistant starch and beta glucan will be fermented into a SCFA pattern high in acetate and that this will lead to beneficial effects on human substrate and energy metabolism, we aim to address the following primary objective: To investigate the effects of an acute administration of inulin/beta glucan in combination with resistant starch on fecal and plasma acetate, as well as on fasting and postprandial substrate and energy metabolism in lean normoglycemic men and obese, prediabetic men.
Interventions
DIETARY_SUPPLEMENTbeta glucan and resistant starch
12g per day the day before the CID Resistant starch 7.5 g
DIETARY_SUPPLEMENTPlacebo
isocaloric maltodextrin before the CID
DIETARY_SUPPLEMENTbeta gluten
12 g beta gluten there day before the CID
Sponsors
Maastricht University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Age
30 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* lean (BMI ≥ 20kg/m2 and ≤ 24.9kg/m2) * normoglycemic * aged 30 - 65 years OR * overweight/obese (BMI ≥ 25kg/m2 and ≤ 34.9kg/m2) * pre-diabetes * aged between 30 - 65 years.
Exclusion criteria
* diabetes mellitus * gastroenterological diseases or major abdominal surgery (allowed i.e.: ---appendectomy, cholecystectomy) * lactose intolerance and other digestive disorders * cardiovascular disease, cancer, liver or kidney malfunction (determined based on ALAT and creatinine levels, respectively) * disease with a life expectancy shorter than 5 years * Use of antibiotics 3 months prior inclusion * Use of probiotics or prebiotics
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Plasma acetate concentrations (microM) | at baseline (before a high-fat mixed meal) |
Secondary
| Measure | Time frame |
|---|---|
| fat oxidation (g/min) will be measured using an open-circuit ventilated hood system (Omnical, Maastricht University, The Netherlands) | at baseline and four hours after a high fat mixed meal |
| carbohydrate oxidation (g/min) will be measured using an open-circuit ventilated hood system (Omnical, Maastricht University, The Netherlands) | at baseline and four hours after a high fat mixed meal |
| Circulating hormone concentrations (Insulin, GLP-1, PYY) | at baseline and four hours after a high fat mixed meal |
| Energy expenditure (kJ/min) will be measured using an open-circuit ventilated hood system (Omnical, Maastricht University, The Netherlands) | at baseline and four hours after a high fat mixed meal |
| Plasma glucose (mmol/L) | at baseline and four hours after a high fat mixed meal |
| Free Fatty Acids (micromol/L) | at baseline and four hours after a high fat mixed meal |
| Breath H2 using (Bedfont EC60 Gastrolyzer, Rochester, UK) | at baseline and four hours after a high fat mixed meal |
| Circulating metabolite concentrations (Glucose, Free Fatty Acids, Triglycerides) | at baseline and four hours after a high fat mixed meal |
Countries
Netherlands
Outcome results
None listed