Obesity, Pre Diabetes
Conditions
Brief summary
Based on our hypothesis that orally administered resistant starch and inulin/beta glucan will be fermented into a SCFA pattern high in acetate and that this will lead to beneficial effects on human substrate and energy metabolism, we aim to address the following primary objective: To investigate the effects of an acute administration of inulin/beta glucan in combination with resistant starch on fecal and plasma acetate, as well as on fasting and postprandial substrate and energy metabolism in lean normoglycemic men and obese, prediabetic men.
Interventions
DIETARY_SUPPLEMENTInulin and Resistant Starch
12g per inulin in combination 7.5 g resistant starch per day the day before the CID Resistant starch 7.5 g
DIETARY_SUPPLEMENTInulin
12g per inulin in combination with maltodextrin per day the day before the CID
DIETARY_SUPPLEMENTPlacebo
isocaloric maltodextrin before test day
Sponsors
Maastricht University Medical Center
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
MALE
Age
30 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* lean (BMI ≥ 20kg/m2 and ≤ 24.9kg/m2) * normoglycemic * aged 30 - 65 years OR * overweight/obese (BMI ≥ 25kg/m2 and ≤ 34.9kg/m2) * pre-diabetes * aged between 30 - 65 years.
Exclusion criteria
* diabetes mellitus * gastroenterological diseases or major abdominal surgery (allowed i.e.: - appendectomy, cholecystectomy) * lactose intolerance and other digestive disorders * cardiovascular disease, cancer, liver or kidney malfunction (determined -based on ALAT and creatinine levels, respectively) * disease with a life expectancy shorter than 5 years * Use of antibiotics 3 months prior inclusion * Use of probiotics or prebiotics
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Plasma acetate concentrations (microM) | At baseline and up to four hours after a high-fat mixed meal of each CID |
Secondary
| Measure | Time frame |
|---|---|
| Fat oxidation (in g/min) using an open-circuit ventilated hood system (Omnical, Maastricht University, The Netherlands) | At baseline and up to four hours after a high-fat mixed meal of each CID |
| Carbohydrate oxidation (in g/min) using an open-circuit ventilated hood system (Omnical, Maastricht University, The Netherlands) | At baseline and up to four hours after a high-fat mixed meal of each CID |
| Energy expenditure (in kJ/min) using an open-circuit ventilated hood system (Omnical, Maastricht University, The Netherlands) | At baseline and up to four hours after a high-fat mixed meal of each CID |
| Circulating metabolite concentrations (Glucose, Free Fatty Acids, Triglycerides) | At baseline and up to four hours after a high-fat mixed meal of each CID |
| Breath H2 using (Bedfont EC60 Gastrolyzer, Rochester, UK) | At baseline and up to four hours after a high-fat mixed meal of each CID |
| Circulating hormone concentrations (Insulin, GLP-1, PYY) | At baseline and up to four hours after a high-fat mixed meal of each CID |
Countries
Netherlands
Outcome results
None listed