Metabolic Disturbance, Metabolism and Nutrition Disorder
Conditions
Keywords
Acetylcarnitine kinetic, Phosphocreatine (PCr) kinetic
Brief summary
This study aims to measure skeletal muscle metabolism in vivo during exercise using the non-invasive Magnetic Resonance Spectroscopy (MRS) technique. Specifically, phosphocreatine (PCr) kinetic and Acetylcarnitine levels will be determined at resting, during exercise and during the recovery post exercise. The target population is adult healthy men, with a wide range of maximal physical capacity.
Detailed description
Skeletal muscle mitochondrial activation during the transition from resting metabolism to exercise may modulated by CrAT activity. Furthermore, dynamic changes in acetylcarnitine during the transition from exercise to resting metabolism (acetylcarnitine off-kinetic) may also reflect the action of CrAT protein. To date, the only possibility to get information on CrAT activity is by taking a muscle biopsy and perform enzyme activity assays ex vivo. By dynamic MRS scanning, it might be possible to deduce information that reflects CrAT activity, thereby making the biopsy sampling unnecessary.
Interventions
OTHERExercise metabolism
Participants will perform a series of exercises inside and outside the MRS/MRI scanner.
Sponsors
Maastricht University
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
MALE
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Males, Generally healthy * Age 18-40 years old * BMI 18 - 25 kg/m2 * No medication use that interfere with the aims of the study * Stable level of daily physical activity or training for at least 6 months
Exclusion criteria
* Females * Regular smokers, drug abuse * Participation in other studies * Weight gain/loss \> 3 kg in the last 6 months * Contraindications for MRS scans: * Vegetarian eating behavior
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Skeletal muscle PCr on-kinetic during exercise and carnitine acetyltransferase (CrAT) protein content/activity | MRS+Exercise test day 2 | The correlation between skeletal muscle PCr on-kinetic during one-legged exercise and CrAT protein activity will be determined. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Skeletal muscle acetylcarnitine recovery after one-legged exercise and carnitine acetyltransferase (CrAT) protein content/activity | MRS+Exercise test day 2 | The correlation between skeletal muscle acetylcarnitine off-kinetic after one-legged exercise and CrAT protein activity will be determined. |
| Skeletal muscle CrAT protein activity, from muscle biopsy, and PCr breakdown rate during one-legged exercise | MRS+Exercise test day 1 | The correlation between CrAT protein activity and PCr hydrolysis/breakdown will be determined |
| Skeletal muscle acetylcarnitine formation capacity upon exercise and total carnitine availability in skeletal muscle | MRS+Exercise test day 2 | The correlation between acetylcarnitine formation upon exercise and total carnitine availability will be determined |
| Skeletal muscle mitochondrial function in vitro | MRS+Exercise test day 2 | The association between Skeletal muscle mitochondrial function in vitro and in vivo |
| Skeletal muscle oxygen availability | MRS test day 1 | in vivo assessment of oxygen availability in muscle tissue upon hypoxic stimulus during resting |
Countries
Netherlands
Outcome results
None listed