Oocyte Competence
Conditions
Keywords
Oocyte, Embryo, Development, Pregnancy, Competence
Brief summary
The present study aims to describe novel non-invasive parameters related to the competence of female reproductive cells (oocytes). In a fertility unit, human reproductive cells and embryos are subjected to different imaging technologies. Images can easily be obtained without disturbing the microenvironment during different standard procedures. Turning images into quantifiable data might provide a further understanding on oocyte behavior and performance in the laboratory.
Interventions
OTHERImage acquisition and analysis
Oocyte image obtention and analysis prior to intracytoplasmic sperm injection
Sponsors
University College, London
Center for Reproductive an Genetic Health
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* Intracytoplasmic sperm injection * Embryo culture with subsequent fresh embryo transfer
Exclusion criteria
\- Conventional IVF
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Implantation | 6 weeks gestation | Number of gestational sacs seen on ultrasound scan |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Embryo quality clevage | 68 ± 1 hours post insemination | Quality after three days of culture |
| Embryo quality Blastocyst | 116 ±2 hours post insemination | Quality at the blastocyst stage |
| Fertilisation | 17 ± 1 hours post insemination | Appearance of two pronucleii after insemination |
| Nuclear maturity | 36-41 post hCG | Presence of meiotic spindle (if available, PolScope) |
| Live Birth | 40 weeks gestation | Baby to be born after embryo transfer |
| Euploidy | 2-3 weeks post insemination | Number of chromosomes (if available, after PGT-A) |
Countries
United Kingdom
Outcome results
None listed