Hepatic Fat Content
Conditions
Keywords
hepatic fat content, dietary fat, glucose, fructose, non-esterified fatty acid, triglyceride, insulin, postprandial period
Brief summary
The first primary research objective of the study is to determine whether high-fat load (150 g of fat) induces increase in hepatic fat content (HFC) three and six hours after meal in insulin-sensitive subjects with normal HFC (\<5% of fat) and in non-diabetic subjects with an increased HFC (\>5% of fat). Furthermore, the other objective of the study is to determine whether the response of HFC to a high-fat load is affected by coadministration of glucose or fructose.
Detailed description
The epidemic of non-alcoholic fatty liver disease (NAFLD) is becoming a major challenge faced by health system worldwide. The hepatic fat comes from the major sources - non-esterified fatty acids (NEFA) released from adipose tissue, dietary fat and de novo lipogenesis. Fat accumulation in the liver then occurs when triglycerides (TG) that are formed are not rapidly enough oxidized or secreted from the liver in very low density lipoproteins (VLDL). It can be hypothesized that the capacity of the liver to eliminate TG can be overcome after high load of dietary fat. High-fat load should induce an immediate accumulation of hepatic fat that could be detected using 1H magnetic resonance spectroscopy (1H-MRS). The accumulation of liver fat can be also affected by coadministration of simple carbohydrates - glucose and fructose. The administration of these carbohydrates can have a pronounced impact on the availability of particular sources of hepatic fat.
Interventions
DIETARY_SUPPLEMENTFat
Subjects will consume dairy cream (150 g of fat) at time 0 hours.
DIETARY_SUPPLEMENTFasting
Subjects will fast throughout the experiment.
DIETARY_SUPPLEMENTFat+Glucose
Subjects will consume dairy cream (150 g of fat) at time 0 hours. At the same time and 2 and 4 hours later they will drink herbal tea containing 50 g of glucose.
DIETARY_SUPPLEMENTGlucose
Subjects will drink herbal tea containing 50 g of glucose at times 0, 2 and 4 hours.
DIETARY_SUPPLEMENTFat+Fructose
Subjects will consume dairy cream (150 g of fat) at time 0 hours. At the same time and 2 and 4 hours later they will drink herbal tea containing 50 g of fructose.
DIETARY_SUPPLEMENTFructose
Subjects will drink herbal tea containing 50 g of fructose at times 0, 2 and 4 hours.
Sponsors
Institute for Clinical and Experimental Medicine
Study design
Allocation
RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
10 non-obese insulin-sensitive male volunteers with normal hepatic fat content (less than 5% of liver fat) and 10 non-obese non-diabetic subjects with increased hepatic fat content (more than 5% of hepatic fat) will undergo series of six experiments - changes of liver fat content will be measured at time 0, 3 and 6 hours after specific dietary interventions
Eligibility
Sex/Gender
MALE
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* normal response to oral glucose tolerance test * normal glycated hemoglobin * normal aspartate aminotransferase (AST) * normal alanine aminotransferase (ALT)
Exclusion criteria
* BMI \> 30 kg/m2 * use of pharmacological agents affecting insulin sensitivity * use of pharmacological agents affecting lipid metabolism * inability to undergo 1H-MRS examination
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Hepatic fat content | Before time 0 hour, 3 hours, 6 hours | Change in hepatic fat content as determined by proton magnetic resonance spectroscopy (%) between 0 and 6 hours |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| TG | before 0, 0.5, 1, 2, 2.5, 3, 4, 4.5, 5, and 6 hours | Plasma triglyceride concentration |
| NEFA | before 0, 0.5, 1, 2, 2.5, 3, 4, 4.5, 5, and 6 hours | Plasma non-esterified fatty-acid concentration |
| Glucose | before 0, 0.5, 1, 2, 2.5, 3, 4, 4.5, 5, and 6 hours | Plasma glucose concentration |
| Insulin | before 0, 0.5, 1, 2, 2.5, 3, 4, 4.5, 5, and 6 hours | Plasma insulin concentration |
| Glucagon | before 0, 0.5, 1, 2, 2.5, 3, 4, 4.5, 5, and 6 hours | Plasma glucagon concentration |
Countries
Czechia
Outcome results
None listed