Antibiotic Drug Concentration in Sweat
Conditions
Keywords
antibiotic drug monitoring in sweat
Brief summary
The secretion of different kinds of antibiotic drugs in sweat is investigated. The concentration and pharmacodynamics of antibiotic drugs in sweat and blood will be compared.
Interventions
DIAGNOSTIC_TESTanalysis of last resort antibiotic drug (Flucloxacillin, Imipenem, Vancomycin or Cefepim) in sweat and blood
blood draw (4.7 ml EDTA) and sweat sample taken (Macroduct Sweat Collecting System)
Sponsors
University Hospital, Basel, Switzerland
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Adult patients * Ability to understand the purpose of the study, provide signed and dated informed consent and qualify for therapeutic drug monitoring * In-patients receiving intravenous therapy with last resort antibiotics Cefepim, Floxapen, Imipenem or Vancomycin for at least 24 hours * Glomerular Filtration Rate \>/= 50 ml/Min
Exclusion criteria
* Glomerular Filtration Rate \< 50 ml/Min
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in detection of last resort antibiotic Cefepim in sweat. | sample taken immediately before Cefepim application and 2 hours, 4 hours, 6 hours, 8 hours after Cefepim application | detection of Cefepim in sweat by mass spectrometry (proof of concept). |
| Change in detection of last resort antibiotic Flucloxacillin in sweat. | sample taken immediately before and 45 minutes after Flucloxacillin application | detection of Flucloxacillin in sweat by mass spectrometry (proof of concept). |
| Change in detection of last resort antibiotic Imipenem in sweat. | sample taken immediately before and 60 minutes after Imipenem application | detection of Imipenem in sweat by mass spectrometry (proof of concept). |
| Change in detection of last resort antibiotic Vancomycin in sweat. | sample taken immediately before and 5 hours after Vancomycin application | detection of Vancomycin in sweat by mass spectrometry (proof of concept). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| concentration (yg/ml) of last resort antibiotic Flucloxacillin in sweat. | sample taken immediately before and 45 minutes after Flucloxacillin application | quantification of Flucloxacillin in sweat by mass spectrometry. |
| concentration (yg/ml) of last resort antibiotic Imipenem in sweat. | sample taken immediately before and 60 minutes after Imipenem application | quantification of Imipenem in sweat by mass spectrometry |
| concentration (yg/ml) of last resort antibiotic Vancomycin in sweat. | sample taken immediately before and 5 hours after Vancomycin application | quantification of Vancomycin in sweat by mass spectrometry |
| concentration (yg/ml) of last resort antibiotic Cefepim in sweat. | sample taken immediately before Cefepim application and 2 hours, 4 hours, 6 hours, 8 hours after Cefepim application | quantification of Cefepim in sweat and blood by mass spectrometry |
Countries
Switzerland
Outcome results
None listed