Infertility
Conditions
Brief summary
Vienna Consensus has identified 10% damage rate after ICSI as a competency value. Despite the highest quality embryologists doing ICSI, degeneration sometimes occurs due to oocyte factors such as the fragile membrane, etc. Cytochalasin B serves to facilitate spindle or pronuclear transfer procedures helping to reduce the damage rate with no harm reported. Using Cytochalasin B during ICSI could serve to rescue some oocytes from the degeneration allowing for more chances of viable zygotes,
Interventions
OTHERCytochalasin B use in ICSI handling medium
A medium with in-house supplementation of Cytochalasin B to decrease oocyte degeneration after ICSI and improve survival rate
Sponsors
Banoon IVF Center
Ibn Sina Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 40 Years
Healthy volunteers
No
Inclusion criteria
* All ICSI candidates patients
Exclusion criteria
* No exclusion
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| oocyte survival rate | 6 days of culture | number of survived oocyte after intracytoplasmic sperm injection |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| fertilization rate | 6 days of culture | number of fertilized oocytes per metaphase II injected |
| blastocyst formation rate | 6 days of culture | number of formed blastocyst per fertilized oocyte |
| embryo utilization rate | 6 days of culture | number of embryo transferred added to number of those cryopreserved per fertilized oocyte |
| clinical pregnancy rate | three months | number of women with a heartbeat at week 4 or more after embryo transfer |
| ongoing pregnancy rate | four months | number of women with continued pregnancy after twelve weeks of gestation |
Countries
Egypt
Outcome results
None listed