Gingival Crevicular Fluid, Neurogenic Inflammation, Periimplant Diseases, Periimplant Sulcular Fluid, Periodontal Diseases
Conditions
Brief summary
Regulatory effects of some neuropeptides substance-P (SP), neurokinin-A (NKA), calcitonin gene-linked peptide (CGRP) and neuropeptide-Y (NPY) )on inflammatory responses in periodontal disease has been described; however, the impact of neuropeptide levels are not clearly defined in healthy and diseased peri-implant tissues.ın order to evaluate the situation, thirty-nine implants that have been loaded over the past 12 months and their symmetrically matching teeth were evaluated using a split-mouth study design. Clinical periodontal examinations included the Silness-Löe plaque index, Löe-Silness gingival index, bleeding on probing, probing pocket depth and clinical attachment level parameters were determined. Gingival crevicular fluid (GCF)/Periimplant sulcular fluid (PISF) samples were collected, and the levels of the neuropeptides were determined by enzyme-linked immunosorbent assay. Correlations between GCF/PISF neuropeptide levels and the clinical examination parameters were evaluated in the peri-implant/periodontal soft tissues.
Interventions
DIAGNOSTIC_TESTgingival crevicular fluid (GCF) and peri-implant sulcular fluid(PISF) collection
GCF/PISF samples were collected using periopaper strips. Prior to sample collection, each site was gently air-dried, all supragingival plaque was removed, and the area was carefully isolated to prevent samples from being contaminated by saliva.
Sponsors
Ondokuz Mayıs University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
MALE
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
The main inclusion criteria for patient selection were as follows: * Compliance with systematic maintenance care; * Adult, non-smoker, systemically healthy * Not using any medicament for the last 6 months ; * Partially dentated patient treated with implant-supported metal ceramic full crowns and/or fixed partial dentures * As the study was split-mouth designed, in each individual at least one implant and one matching tooth in the same mouth symmetrically representing the same tooth group (premolars/molars); * A single type of metal ceramic implant restoration that is functional for at least 1 year; and a matching tooth with no dental or endodontic restorations.
Exclusion criteria
* Medical history of cancer, rheumatoid arthritis, diabetes mellitus, or cardiovascular disease and any other systemic disease affecting lipid metabolism (i.e. impaired glucose tolerance, metabolic syndrome); * Compromised immune system; * Pregnancy, menopause, or lactation; * Ongoing drug therapy that might affect the clinical characteristics of periodontitis and lipid metabolism; * Dental treatment during the 6 months prior to data collection.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Gingival crevicular fluid/peri-implant sulcular fluid Level of Substance-P | 8-10 am on the day following periodontal status assessment. | Substance-P (SP) is a neuropeptide of the tachykinin family and marker of neurogenic inflammation. |
| Gingival crevicular fluid/peri-implant sulcular fluid Level of neurokinin-A | 8-10 am on the day following periodontal status assessment. | Neurokinin-A is a neuropeptide of the tachykinin family and marker of neurogenic inflammation. |
| Gingival crevicular fluid/peri-implant sulcular fluid Level of Calcitonin gene-related peptide | 8-10 am on the day following periodontal status assessment. | Calcitonin gene-related peptide (CGRP), another neuropeptide derived from peptidergic nerves and a marker of neurogenic inflammation. |
Outcome results
None listed