Healthy
Conditions
Brief summary
Nucleotides are the building blocks for a variety of molecules, including ATP, which is broken down in the skeletal muscle cells to provide fuel for exercise. Nucleotides can be obtained from the diet, and produced or salvaged by the body. Though nucleotides provide the building blocks for ATP, the sugar ribose has been suggested to be rate limiting for synthesising ATP. Previous studies have shown a decrease in ATP levels in the muscle after intense exercise, and this is linked to fatigue. Other studies have also reported benefits of nucleotide and/or ribose supplementation on exercise performance. However, any potential link between ATP metabolism and exercise performance has not been explored. This study will look at the effects of nucleotide and nucleotide-ribose supplementation in fuel utilisation and performance in skeletal muscle during endurance exercise.
Interventions
DIETARY_SUPPLEMENTLow nucleotide
2 week, twice daily, supplementation with a nucleotide-depleted mycoprotein drink, with added dextrose
DIETARY_SUPPLEMENTHigh nucleotide
2 week, twice daily, supplementation with a nucleotide-rich mycoprotein drink, with added dextrose
DIETARY_SUPPLEMENTHigh nucletide + Ribose
2 week, twice daily, supplementation with a nucleotide-rich mycoprotein drink, with added ribose
Sponsors
University of Exeter
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* BMI between 18 and 30
Exclusion criteria
* On medication (except contraception) * Smokers * Diagnosed with any metabolic or cardiovascular conditions * Muscle or bone injuries * BMI \< 18 or \> 30
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Changes in muscle ATP concentrations | After each supplementation cycle (day 15 of each cycle): baseline (fasted and before exercise), immediately after glycogen depleting exercise (after exercising for 1:30 - 2 hours), immediately after 15 minute time trial and after 3-hour recovery |
Secondary
| Measure | Time frame |
|---|---|
| Changes in muscle glycogen concentrations | After each supplementation cycle (day 15 of each cycle): baseline (fasted and before exercise), immediately after glycogen depleting exercise (after exercising for 1:30 - 2 hours), immediately after 15 minute time trial and after 3-hour recovery |
| Blood lactate concentrations | After supplementation cycle (day 15): baseline and at 15 minute intervals up 1 hour during glycogen depleting exercise |
| Blood glucose concentrations | After supplementation cycle (day 15): baseline and at 15 minute intervals up 1 hour during glycogen depleting exercise |
| Serum uric acid concentrations | Measured at baseline and of days 6, 8 and 15 of each supplementation cycle |
Countries
United Kingdom
Outcome results
None listed