Transplant; Failure, Liver
Conditions
Brief summary
Aims： 1. Conduct multi-center, prospective and open research on liver quality assessment and establish LSCM liver quality assessment system 2. Classification of liver quality grades by LSCM for different pathological conditions (steatosis, fibrosis, hepatocyte necrosis, etc.) 3. Compare the specificity and sensitivity of LSCM evaluation results with the results of liver donor quality evaluation and intraoperative pathological biopsy results. 4. LSCM classifies the quality of donor liver and compares the recovery of liver function on the first day after liver transplantation. 5. LSCM grades the quality of donor liver and compares the long-term prognosis
Interventions
DIAGNOSTIC_TESTLSCM examination
Cut a piece of donor liver and conduct the LSCM examination after staining fluorescein
Sponsors
Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine
Study design
Observational model
CASE_ONLY
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
* All donor livers to be used for liver transplantations
Exclusion criteria
* Exclusion of multiple organ transplantation * Exclusion of detached transplantation and partial liver transplantation, etc. * Removal of blood type incompatible patients for transplantation * Exclusion of poor donor liver quality due to surgical technique (such as hepatic artery embolization) , portal vein thrombosis, etc.) * Any form of substance abuse, mental illness or any disease that the investigator believes may interfere with the patient's understanding of the research requirements. * Can not complete routine research follow-up, and other circumstances that the investigator believes are not suitable for the experiment.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| LSCM examination results | 1 day | LSCM examination is focus on the detections of the hepatocyte nucleus and the micro structures(such as the micro-circulation、central vein and so on). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| HE section staining pathological examination | 1 week | All of donor livers must be detected by HE section staining pathological examination and it is mainly focused on the detection of hepatocyte nucleus and the micro structures(such as the micro-circulation、central vein and so on). |
Other
| Measure | Time frame | Description |
|---|---|---|
| Clinical examinations | 1 week | Mainly focus on the examinations such as ALT、AST、LDH and so on. |
Countries
China
Contacts
Primary ContactZhong Lin, Doctor
Outcome results
None listed