Impact of Oocytes With CLCG on ICSI Outcomes and Their Potential Relation to Pesticide Exposure

Conditions

Pesticide Poisoning

Keywords

oocyte, ovarian stimulation, dysmorphic cytoplasm

Brief summary

Oocyte quality is a key limiting factor in female fertility which is primarily reflected in morphological features. Centrally located cytoplasm granulation (CLCG) is one type of cytoplasmic dimorphism exhibited by oocytes that could be linked to pesticide exposure with a significant risk of decreased ICSI outcomes. This retrospective study included 633 women who were part of an intracytoplasmic spermatozoa injection (ICSI) program between 2009 and 2011. The participants lived in the Picardy region of France and had been exposed to pesticides. The participants were divided in two groups based on prevalence of oocytes with CLCG (LCLCG \[n= 83\]: low prevalence of oocytes with CLCG under 25%. HCLCG \[ n= 68\]: high prevalence of CLCG over 75%). The embryological and clinical outcomes were analysed for both groups and were calculated using the difference between the two values.

Detailed description

Oocyte quality is a key limiting factor in female fertility which is primarily reflected in morphological features. Centrally located cytoplasm granulation (CLCG) is one type of cytoplasmic dimorphism exhibited by oocytes that could be linked to pesticide exposure with a significant risk of decreased ICSI outcomes. This retrospective study included 633 women who were part of an intracytoplasmic spermatozoa injection (ICSI) program between 2009 and 2011. The participants lived in the Picardy region of France and had been exposed to pesticides. The participants were divided in two groups based on prevalence of oocytes with CLCG (LCLCG \[n= 83\]: low prevalence of oocytes with CLCG under 25%. HCLCG \[ n= 68\]: high prevalence of CLCG over 75%). The embryological and clinical outcomes were analysed for both groups and were calculated using the difference between the two values. The included population in the investigator's retrospective study consisted of 633 couples who attended a reproductive medical center and who were part of an ICSI program between 2009 and 2011.

Related papers

No related papers found yet.

Interventions

None listed

Study design

Observational model

CASE_ONLY

Time perspective

RETROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

18 Years to No maximum

Healthy volunteers

No

Inclusion criteria

* couples who attendes a reproductive medical center * couples that were part of a ICSI program between 2009 and 2011 * women were normo-responders * female \> 18 years \<35 yeas old

Exclusion criteria

* female \< 18 years \> 35 yeas old * couple with severe male infertility factors on oocyte activation and subsequent embryo development

Design outcomes

Primary

Measure	Time frame	Description
ICS outcomes of couples presenting differetn CLCG prevalence	2years	The aim of the present study was to investigate the ICSI outcomes of couples presenting different CLCG prevalence's of retrieved oocytes (low prevalence of CLCG; fewer than 25% \[LCLCG\] and high prevalence of CLCG; over 75% \[HCLCG\]) and its correlation to pesticide exposure zones in the region of Picardy, France.

Countries

France

Contacts

PRINCIPAL_INVESTIGATORMoncef Ben Khalifa, PU-PH

CHU Amiens-Picardie

Outcome results