Chorioamnionitis, Placenta Diseases, Preterm Infant, Thrombocytopenia
Conditions
Brief summary
Objective:The purpose of this study was to explore the effect and mechanism of maternal chorioamnionitis on placental microvasculature and platelet activation among preterm infants by activating Wnt-Flt1 signal pathway . Methods:With clinical randomized controlled trial (RCT), the cases were matched with 1:1 according to gestational age and divided into 2 groups according to the placental pathology result: chorioamnionitis group and control group. (1) To observe the platelet parameter, birth weight, thrombrocytopenia and hemorrhage complication, such as intracranial hemorrhage, retinal hemorrhage, pulmonary hemorrhage and gastrointestinal hemorrhage. (2) To observe the miscrovascular density (MVD) in placenta, platelet activating factor (CD62p,CD63) and thrombopotetin (TPO) in preterrn infants.The placental MVD was assessed by immunohistochemical method. The platelet activating factors were detected by flow cytometry. TPO was detected by ELISA. (3) To observe Wnt5a, Flt1 and VEGF in placenta and fetal circulation.The measurement data were analyzed by pair t test and conditional logistic regression. Pearson correlation analysis was used for relationship.
Detailed description
The exclusive criteria included :(1) born to mother with no any other maternal complications; (2) no congenital abnormalities and neonatal asphyxia; (3) died or discharged within 72 hours.
Interventions
OTHERchorioamnionitis
It is a observation research. We didn't intervene anything. The chorioamnionitis groups included the infants whose mothers were diagnosed chorioamnionitis by placental histopathology.
Sponsors
Guangdong Women and Children Hospital
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
1 Hours to No maximum
Healthy volunteers
Yes
Inclusion criteria
1. gestational age\<37weeks 2. admitted to the NICU at Guangdong Women and Children Hospital, with a date of birth from June 2016 to December 2016 3. whose mothers were diagnosed chorioamnionitis by placental histopathology
Exclusion criteria
1. born to mother with no any other maternal complications 2. no congenital abnormalities and neonatal asphyxia; 3. died or discharged within 72 hours.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Placental Microvascular Density(%) | 24 hours | Placental Microvascular Density (by placental histopathology) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Platelet counts (PLT) | 24 hours | PLT (10\^9/L) |
| Thrombopoietin (cord blood) | 24 hours | TPO(ng/ml) |
| Platelets Activation Factors in cord blood | 24 hours | CD62p(%) |
| Flt1 in Placenta(IOD) | 24 hours | by placental histopathology |
| VEGF in Placenta(IOD) | 24 hours | by placental histopathology |
| platelet distribution width(PDW) | 24 hours | PDW (%) |
| Incidence Hemorrhage Disease (%) | through study completion, an average of half year | intraventricular hemorrhage (IVH), retinal hemorrhage, pulmonary hemorrhage and gastrointestinal hemorrhage |
| Wnt5a in Placenta(IOD) | 24 hours | by placental histopathology |
| mean platelet volume(MPV) | 24 hours | MPV (Fl) |
| Flt1 in infants (ng/ml) | 24 hours | test them by Elisa from cord blood |
| VEGF in infants (ng/ml) | 24 hours | test them by Elisa from cord blood |
| Wnt5 in infants (ng/ml) | 24 hours | test them by Elisa from cord blood |
Outcome results
None listed