Exercise
Conditions
Brief summary
The purpose of this graduate student research study is to investigate the effects of nitrate from beetroot powder with additional anthocyanins from tart cherry on exercise performance in healthy Loma Linda University students utilizing the Wingate Anaerobic test on stationary bike.
Detailed description
A randomized controlled double-blind crossover study with a baseline and two interventions. On day 1 (baseline), all subjects will have a lactate fingerstick measurement before and after the Wingate protocol. A random subsample (1/3 of subjects) will have 10 milliliters (2 teaspoon) of blood drawn for determination of serum allantoin. Subjects will then be equipped with a pulse oximeter and a heart rate monitor after which they will perform the Wingate Anaerobic test protocol. The protocol includes: 4 minutes of warm up, 3 seconds of warm up sprint with weights, 1-minute relaxed cycling, 2-minute rest, then 10 seconds cycling without weights. This will be followed by 30 seconds sprint with weights and end with 2 minutes of relaxed cycling. A post-activity blood draw will be performed for another 10 milliliters (2 teaspoon) of blood from the same one-third of subjects previously sampled. After baseline data collection, subjects will undergo a 1-week washout (avoiding high antioxidant foods) after which they will be randomized to treatment groups. On day 9, subjects will begin supplementation with nitrate from beetroot powder with additional anthocyanins, or nitrate from beetroot powder with a placebo for 7 days. On day 15 the subject will again repeat the same procedures as day 1, followed by another week of washout. They will supplement again on day 23 and repeat the same procedure as the first intervention on day 29.
Interventions
OTHERbeetroot and anthocyanin
Beetroot and anthocyanin will be taken for 7 days during the study two hours prior to eating.
OTHERbeetroot and placebo
Beetroot and placebo will be taken for 7 days during the study two hours prior to eating.
Sponsors
Loma Linda University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
OTHER
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
21 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy Loma Linda University students aged between 21 and 35 years old * Willing to abstain from caffeine, mouthwash, chewing gums, and high antioxidant foods during the week of supplementation.
Exclusion criteria
* Diabetic * having salivary gland disorder, cardiovascular disease or orthopedic injuries * pregnant or breastfeeding * taking NSAIDS * have an allergy to anthocyanins and/or beetroot * women who are on their menses
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in blood lactate levels | Day 1 | Participants in this study should reach an anaerobic state as they perform the Wingate test. This lactate pro analyzer will be a tool to examine the participant's change in blood lactate levels by means of POC fingertip lactate measurement. Lactate levels will be measured in all participants at baseline, before and after Wingate Anaerobic test. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Allantoin blood level | Baseline, day 15, and day 29 | Non-fasting blood tests will be done for allantoin during baseline, intervention I, and intervention II. |
Countries
United States
Outcome results
None listed