Sequential Versus Single O2 Levels on Human Embryo Development

5% O2 or Lower is the Ideal Tension for Culturing Human Embryo Development From Day 3 Onward

Status

Completed

Phases

Study type

Interventional

Source

ClinicalTrials.gov

Registry ID

NCT03630900

Enrollment

487

Registered

2018-08-15

Start date

2018-08-24

Completion date

2019-07-01

Last updated

2019-07-16

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Embryo Culture

Brief summary

Myriads of elements affect human embryo culture in vitro. These include, but not limited to, air quality, culture media, culture temperature, incubator humidity, pH, and O2 levels. O2 tension has been thoroughly investigated and, to date, 5% O2 level is superior to 20%. Reports showed that lowering the O2 from Day 3 of embryo culture seems to be physiological.

Interventions

OTHERSequential O2 Culture

We investigate the effect of sequential O2 levels on human embryo culture after Intracytoplasmic sperm injection (ICSI)

Study design

Allocation

RANDOMIZED

Intervention model

PARALLEL

Primary purpose

OTHER

Masking

DOUBLE (Subject, Outcomes Assessor)

Eligibility

Sex/Gender

FEMALE

Age

18 Years to 40 Years

Healthy volunteers

Inclusion criteria

* ICSI cycles undergoing their first ICSI attempt

Exclusion criteria

* ICSI cycles with a previous failure

Design outcomes

Primary

Measure	Time frame	Description
Ongoing Implantation rate	three months	number of sacs with a heartbeat after week twelve of gestation

Secondary

Measure	Time frame	Description
clinical and ongoing pregnancies	three months	registered pregnancy with fetal heartbeat at week four after transfer and after week twelve of gestation
human embryo development in vitro	three months	—

Countries

Egypt

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026