Health, Subjective
Conditions
Keywords
rapeseed, protein, postprandial, glucose
Brief summary
The aim of the study is to investigate the effect of rapeseed proteins on the postprandial metabolic response. Therefore, study participants will receive a meal rich in fat and carbohydrates with and without rapeseed protein or with soy protein that serves as control.
Detailed description
The current study is a monocentric, controlled intervention study that will be conducted as cross-over design. Three interventions are planned and participants will be invited to consume a test meal with added rapeseed protein isolate, a test meal with added soy protein isolate and a test meal without additional protein. The evening before, the subjects will receive a standardized dinner. After a 12-hour fasting overnight, a cannula will be placed. Blood samples will be taken before and after eating the test meal rich in carbohydrate and fat. The test meal consists of pasta with oily tomato sauce with either 25 g of rapeseed protein, 25 g of soy protein or no additional protein. A total of 11 blood samples will be collected over a period of 6 hours. In the meantime, the blood pressure and pulse will be measured repeatedly and information on satiety will be recorded. The interventions will be interrupted by a period of, at least, 2 weeks.
Interventions
DIETARY_SUPPLEMENTrapeseed or soy protein or no protein
25g rapeseed protein in comparison to 25g soy protein or no protein - dosage of 25 g per day
Sponsors
UFOP Union for the promotion of oil and protein plants eV
University of Halle Medical Faculty
Martin-Luther-Universität Halle-Wittenberg
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* healthy men and women * age between 18 and 65 years * informed consent * body mass index between 18,5 up to 29,9 kg/m2 * anamnesis without diagnostic findings
Exclusion criteria
* acute or chronic diseases ((hypertension, coronary diseases, renal diseases, diabetes, alcohol abuse etc.) * intake of drugs (exemption: oral contraceptive) * pregnancy or lactation * food intolerance or allergies to mustard, soy, tomato, wheat * participation on another trial * blood donation - while last 2 month before the start of the study * extensive physical activity (competitive sports, hard physical work) * diet procedure * smoking
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| time course of glucose level | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | Impact of rapeseed protein on the postprandial blood glucose level |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| triglycerides | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | Impact of rapeseed protein on the postprandial levels of triglycerides |
| short chain fatty acids | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | Impact of rapeseed protein on the postprandial levels of short chain fatty acids |
| indole-3-propionate | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | Impact of rapeseed protein on the postprandial levels of indole-3-propionate |
| amino acids | 0,15,30,45,60,90,120 and 180 min after ingestion of testmeal rich in carbohydrates and fat | Impact of rapeseed protein on the postprandial levels of amino acids |
| hormones (insulin, ghrelin, FGF23) | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | Impact of rapeseed protein on the postprandial levels of hormones (insulin, ghrelin, FGF23) |
| Cholesterol | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | Impact of rapeseed protein on the postprandial levels of Cholesterol |
| minerals and blood hsCRP | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | Impact of rapeseed protein on the postprandial levels of minerals and blood hsCRP |
| systolic and diastolic blood pressures | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | time course: measurement of blood pressure in mm Hg |
| feeling of satiety by means of a questionnaire | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | — |
| heart rate | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | time course: measurement of heart rate in beats per minute |
| time course of urea level, | 0,15,30,45,60,90,120,180,240,300 and 360 min after ingestion of testmeal rich in carbohydrates and fat | Impact of rapeseed protein on the postprandial levels of urea |
Countries
Germany
Outcome results
None listed