Gut Microbiota, Metabolic Syndrome
Conditions
Keywords
Raspberry, Diet
Brief summary
There is growing evidence that nutritional intervention with dietary polyphenols can positively modulate the gut microbiota to improve cardiometabolic health. Whether the beneficial effects of raspberry on obesity and the metabolic syndrome can be linked to their potential impact on the gut microbiota and intestinal integrity remains speculative at this time. Moreover, the mechanisms of action underlying health benefits associated to raspberry consumption are still unknown. The investigators are thus proposing to combine the study of metagenomics, transcriptomics and metabolomics to test whether a prebiotic activity of raspberry can play a role in the prevention of obesity-linked metabolic syndrome in a clinical setting.
Interventions
DIETARY_SUPPLEMENTRaspberry supplement
During the 8-week protocol, participants will be invited either to consume 280g of frozen raspberries daily (morning and evening). The daily dose is equivalent to 2 cups of raspberries.
Sponsors
Laval University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Men and premenopause women in good health * Caucasians * At least one of the following : BMI between 25 and 40 kg/m2 or Waist circumference ≥ 80 cm for women and ≥ 94 cm for men * At least one of the following : TG ≥ 1.35 mmol/L or fasting insulinemia ≥ 42 pmol/L
Exclusion criteria
* Metabolic disorders (hypertension, diabetes, hypercholesterolemia) * Regular use of medication affecting study parameters * Use of natural health product in the last 3 months * Use of antibiotics in the last 3 months * Nicotine users * Allergy or intolerance for raspberries * Raspberry taste aversion * More than 2 alcohol drinks par day * Particular dietary habits (vegetarism, gluten-free diet, cetogenic diet...) * Weight change of more than 5% in the last 3 months * Surgery in the last 3 months or planed during the study
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Changes in plasma insulin/glucose of overweight/obese men and women taking frozen raspberries daily or following their usual diet (control group). | 12 months |
| Changes in plasma lipides/lipoproteins of overweight/obese men and women taking frozen raspberries daily or following their usual diet (control group). | 12 months |
Secondary
| Measure | Time frame |
|---|---|
| Changes in gene expression in overweight/obese men and women taking frozen raspberries daily or following their usual diet (control group). | 3-6 months |
| Changes in metabolites concentration in overweight/obese men and women taking frozen raspberries daily or following their usual diet (control group). | 3-6 months |
| Changes in the gut microbiota composition in overweight/obese men and women taking frozen raspberries daily or following their usual diet (control group). | 3-6 months |
| Changes in systolic and diastolic blood pressure of overweight/obese men and women taking frozen raspberries daily or following their usual diet (control group). | 12 months |
| Changes in intestinal integrity (plasma lipopolysaccharide (LPS) and LPS binding protein (LBP) will be measured) of overweight/obese men and women taking frozen raspberries daily or following their usual diet (control group). | 12 months |
| Changes in inflammation biomarkers of overweight/obese men and women taking frozen raspberries daily or following their usual diet (control group). | 12 months |
Countries
Canada
Contacts
PRINCIPAL_INVESTIGATORMarie-Claude Vohl
Laval University
Outcome results
None listed