TSHβX1 and D2 THR92ALA in Pregnancy

TSHβX1 Splice Variant Expression and D2 Thr92Ala Polymorphism Analysis in Pregnant Women With Thyroid Diseases

Status

Completed

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT03612908

Enrollment

Registered

2018-08-02

Start date

2016-09-01

Completion date

2018-06-30

Last updated

2018-08-02

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Hyperthyroidism, Hypothyroidism, Pregnancy Related

Keywords

Hyperthyroidism, Polymorphism, TSHβX1, D2 THR92ALA, Hypothyroidism

Brief summary

Thyroid diseases are pathologies that frequently affect pregnant women causing serious complications. This current research aims to find out whether the expression of TSHβX1 splice variant and D2 Thr92Ala polymorphism in the DIO2 gene are associated with thyroid disease in Mexican pregnant women.

Detailed description

Deiodinase 2 (DIO2) is a selenoenzyme responsible for the deiodination of T4 to T3, which makes it crucial for the proper functioning of thyroid hormones. Polymorphisms of DIO2 alters the enzymatic function. In addition, the Ala92Ala genotype was reported to be related with a reduction in the placental activity of D2, which could worsen gestational complications. In the other hand, the TSHβ gene (NC\_000001.11) located on chromosome 1 at position 38p12, codes for the beta unit of the thyroid stimulating hormone (TSH). It is admitted that genetic variants can show a different spectrum of actions. This was a clinical, comparative, prospective and transversal study. Pregnant women aged 18-44 years old, were invited to identify it they had a DIO2 polymorphism or the TSHβX1 splice variant.

Interventions

None listed

Study design

Observational model

CASE_CONTROL

Time perspective

PROSPECTIVE

Eligibility

Sex/Gender

FEMALE

Age

18 Years to 44 Years

Healthy volunteers

Yes

Inclusion criteria

* Pregnant women attended at the Mónica Pretelini Sáenz Maternal-Perinatal Hospital (HMPMPS), Health Institute of the State of Mexico (ISEM), Toluca, Mexico.

Exclusion criteria

* Patients with chronic diseases other than hyper or hypothyroidism. * Patients that required attention in the obstetric intensive care unit.

Design outcomes

Primary

Measure	Time frame	Description
TSHβX1 splice variant expression.	Baseline.	Frequency of positive cases for the TSHβX1 splice variant identified by real-time polymerase change reaction.

Secondary

Measure	Time frame	Description
D2 Thr92Ala polymorphism.	Baseline.	Allele frequency identification of the D2 Thr92Ala polymorphism by genotyping method.

Countries

Mexico

Outcome results