Nitric Oxide, Oral Soft Tissue Conditions, Vasodilation
Conditions
Keywords
spreading vasodilation, gingival microcirculation
Brief summary
The aim of this study is to observe the mechanism of spreading vasodilatation on human healthy gingiva. Nitric-oxide donor solutions in lower and higher concentration are used to trigger the vasoactivity.
Detailed description
Spatial regulation of gingival microcirculation has not been revealed yet. Although this could be an important mechanism to protect gingiva as it is exposed to mechanical, chemical, thermal etc. irritation during whole life. It is especially important during flap surgery. Laser Speckle Contrast Imaging (LSCI) is novel non-invasive method with high spatio-temporal resolution, therefore it allows us to study remote effect of local vasodilation such as spreading vasodilation in human gingiva. The vasodilatator nitric-oxide (NO) donor will be used to trigger remote effect on gingiva. NO donor solutions will be applied in 1 mg/ml and 8 mg/ml nitroglycerin concentrations. Solution will be dropped on the gingiva at tooth 12 in a fabricated leakage proof well attached to 2mm above the marginal gingiva. The change on blood flow will be monitor at the test well with surrounding gingival area spanning 3 tooth and at the contralateral side by LSCI.
Interventions
DRUGnitroglycerin
Vasodilatator solution is dropped on the gingiva.
Sponsors
Semmelweis University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Subject)
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* general health * minimum 5 mm keratinized gingiva at upper front teeth
Exclusion criteria
* pregnancy and breast-feeding * medication (except contraceptives) * smoking * periodontitis * caries * insufficient marginal integrity
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blood flow change after application of NO agonist | 30 min | The change in blood flow will be measured after locally applied nitroglycerin at the site fo application and at the remote sites |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Comparsion of the change in blood flow between genders | 30min | The maximal blood flow changed assessed in primary outcome will be split into two groups based on gender. The change will be compared between males and females. |
| Correlation between the change in blood flow and gingival thickness | 30min | The biotpye will be assessed by measurement of gingival thickness by an ultrasonic devices. The correlation between thickness and maximal vasodilation will be evaluated. |
Countries
Hungary
Outcome results
None listed