Septicemia
Conditions
Keywords
Microarray Assay Technique, Mass Spectrometry Technique, Time to appropriate antibiotic
Brief summary
This is an experimental study of participants who had positive blood culture to compare time to appropriate antibiotic between using Microarray Assay and Mass Spectrometry to bacterial Identification.
Detailed description
Between June to Dec 2018, 200 participants those met the eligibility criteria were enrolled in this study that is a quasi experimental study. All participants were empirical antibiotic therapy by infectious physician. 100 patients in Pre-intervention group had bacterial identification by Mass spectrometry technique from bacterial colony and 100 patients in Post-intervention group had bacterial identification by Microarray assay from blood culture. Antibiotic adjustment were considered after bacterial identification and result of antibiotic susceptibility testing. Then all participants were re-evaluated outcome after treatment. Finally, the results were ready to analysis in January 2019.
Interventions
DIAGNOSTIC_TESTMass spectrometry
Molecular diagnostic test for bacterial identification from bacterial colony
DIAGNOSTIC_TESTMicroarray assay
Bacterial identification by Microarray assay from positive blood culture and confirmed by Mass spectrometry technique from bacterial colony. Adjusted antibiotic therapy according to AST Re-evaluation after treatment
Sponsors
Chiang Mai University
Study design
Allocation
NON_RANDOMIZED
Intervention model
SEQUENTIAL
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Age ≥ 18 year old with positive hemoculture between June 2018 and Dec 2018 * Internal medicine wards patients at Maharaj Nakorn Chiang Mai Hospital * Ability to provide informed consent
Exclusion criteria
* Suspected contaminated positive blood culture * Bacteremia cause by more than two species of bacteria, as suspected from the gram staining examinations * Terminally ill patients
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| To compare the time to appropriate antibiotic therapy in patients with positive blood cultures between using Microarray assays versus Mass Spectrometry technique for Identification of Positive Blood Cultures | 4 days from positive hemoculture | Comparison of time that participants received appropriate antibiotic therapy between 2 study period (Mass spectrometry technique and Microarray assay group) |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Mortality rate at 2 weeks | 14 days from positive hemoculture | To compare proportion of mortality between 2 study periods (Mass spectrometry technique and Microarray assay group) at 2 weeks after enrollment |
| Length of hospital stays | 30 days | To compare lengths of hospital stay between 2 study periods (Mass spectrometry technique and Microarray assay group) |
| Cost of antibiotic therapy | 14 days | To compare the cost of antibiotics for the course of treatment during study period between 2 study period (Mass spectrometry technique and Microarray assay group) |
| Percentage of De-escalation or escalation antibiotic | 14 days | To compare percentage of De-escalation or escalation antibiotic between 2 study periods (Mass spectrometry technique and Microarray assay group) |
Countries
Thailand
Contacts
Primary ContactParichart Sakulkonkij, MD
Backup ContactParichat Salee, MD
Outcome results
None listed